Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves

Servan de Almeida Renata, Spilki Fernando Rosado, Roehe Paulo Michel, Arns Clarice Weis. 2005. Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves. Veterinary Microbiology, 105 (2) : 131-135.

Résumé : A reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emphysema, and lung consolidated areas. Lung and tracheal tissues collected 6 days after infection were analysed by RT-nested-PCR. Primers, specific for the BRSV G and F glycoproteins genes, yielded amplification fragments of 371 and 481 bp, respectively, from the RNA of the cell-propagated virus. Using RNA extracted from organs of infected calves, RTnested- PCR amplified the fragment of the G gene in all tracheal samples, but in only two of three lung samples analysed. These results suggest that RT-nested-PCR could be a promising assay for diagnosis and epidemiological analysis of BRSV in Brazil.

Mots-clés géographiques Agrovoc : Brésil

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Servan de Almeida Renata, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Spilki Fernando Rosado, UNICAMP (BRA)
• Roehe Paulo Michel, Institute of Basic Health Sciences (BRA)
• Arns Clarice Weis, UNICAMP (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/568799/)

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