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Variations in type III effector repertoires, pathological phenotypes and host range of Xanthomonas citri pv. citri pathotypes

Escalon Aline, Javegny Stéphanie, Vernière Christian, Noel Laurent D., Vital Karine, Poussier Stéphane, Hajri Ahmed, Boureau Tristan, Pruvost Olivier, Arlat Mathieu, Gagnevin Lionel. 2013. Variations in type III effector repertoires, pathological phenotypes and host range of Xanthomonas citri pv. citri pathotypes. Molecular Plant Pathology, 14 (5) : pp. 483-496.

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : The mechanisms determining the host range of Xanthomonas are still undeciphered, despite much interest in their potential roles in the evolution and emergence of plant pathogenic bacteria. Xanthomonas citri pv. citri (Xci) is an interesting model of host specialization because of its pathogenic variants: pathotype A strains infect a wide range of Rutaceous species, whereas pathotype A*/AW strains have a host range restricted to Mexican lime (Citrus aurantifolia) and alemow (Citrus macrophylla). Based on a collection of 55 strains representative of Xci worldwide diversity assessed by amplified fragment length polymorphism (AFLP), we investigated the distribution of type III effectors (T3Es) in relation to host range. We examined the presence of 66 T3Es from xanthomonads in Xci and identified a repertoire of 28 effectors, 26 of which were shared by all Xci strains, whereas two (xopAG and xopC1) were present only in some A*/AW strains. We found that xopAG (=avrGf1) was present in all AW strains, but also in three A* strains genetically distant from AW, and that all xopAG-containing strains induced the hypersensitive response (HR) on grapefruit and sweet orange. The analysis of xopAD and xopAG suggested horizontal transfer between X.?citri pv. bilvae, another citrus pathogen, and some Xci strains. A strains were genetically less diverse, induced identical phenotypic responses and possessed indistinguishable T3E repertoires. Conversely, A*/AW strains exhibited a wider genetic diversity in which clades correlated with geographical origin and T3E repertoire, but not with pathogenicity, according to T3E deletion experiments. Our data outline the importance of taking into account the heterogeneity of Xci A*/AW strains when analysing the mechanisms of host specialization. (Résumé d'auteur)

Mots-clés Agrovoc : Xanthomonas campestris citri, Citrus aurantiifolia, Citrus macrophylla, Citrus, Pouvoir pathogène, Pathotype, Plante hôte, Phénotype

Mots-clés géographiques Agrovoc : Réunion

Mots-clés complémentaires : Xanthomonas citri pv citri

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Escalon Aline, CIRAD-BIOS-UMR PVBMT (REU)
  • Javegny Stéphanie, CIRAD-BIOS-UMR PVBMT (REU)
  • Vernière Christian, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-2312-2073
  • Noel Laurent D., INRA (FRA)
  • Vital Karine, CIRAD-BIOS-UMR PVBMT (REU)
  • Poussier Stéphane, Université de la Réunion (REU)
  • Hajri Ahmed, INRA (FRA)
  • Boureau Tristan, Université d'Angers (FRA)
  • Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)
  • Arlat Mathieu, Université Toulouse III Paul Sabatier (FRA)
  • Gagnevin Lionel, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/569010/)

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