Montero-Vagas Josaphat Miguel, González-González Lindbergh Humberto, Gálvez-Ponce Eligio, Ramírez-Chávez Enrique, Molina-Torres Jorge, Chagolla Alicia, Montagnon Christophe, Winkler Robert. 2013. Metabolic phenotyping for the classification of coffee trees and the exploration of selection markers. Molecular BioSystems, 9 (4) : 693-699.
Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY
Résumé : High-throughput metabolic phenotyping is a challenge, but it provides an alternative and comprehensive access to the rapid and accurate characterization of plants. In addition to the technical issues of obtaining quantitative data of plenty of metabolic traits from numerous samples, a suitable data processing and statistical evaluation strategy must be developed. We present a simple, robust and highly scalable strategy for the comparison of multiple chemical profiles from coffee and tea leaf extracts, based on direct-injection electrospray mass spectrometry (DIESI-MS) and hierarchical cluster analysis (HCA). More than 3500 individual Coffea canephora and Coffea arabica trees from experimental fields in Mexico were sampled and processed using this method. Our strategy permits the classification of trees according to their metabolic fingerprints and the screening for families with desired characteristics, such as extraordinarily high or low caffeine content in their leaves.
Mots-clés Agrovoc : Coffea canephora, Coffea arabica, phénotype, classification, marqueur génétique, spectrométrie de masse
Mots-clés géographiques Agrovoc : Mexique
Classification Agris : F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
- Montero-Vagas Josaphat Miguel, Cinvestav (MEX)
- González-González Lindbergh Humberto, Instituto Tecnologico de Tuxtepec (MEX)
- Gálvez-Ponce Eligio, Agroindustrias Unidas de México (MEX)
- Ramírez-Chávez Enrique, Cinvestav (MEX)
- Molina-Torres Jorge, Cinvestav (MEX)
- Chagolla Alicia, Cinvestav (MEX)
- Montagnon Christophe, CIRAD-BIOS-UMR RPB (FRA)
- Winkler Robert, Cinvestav (MEX)
Source : Cirad - Agritrop (https://agritrop.cirad.fr/569012/)
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