Interaction between environmental factors affects the accumulation of root proteins in hydroponically grown Eucalyptus globulus (Labill.)

Bedon Franck, Majada Juan, Feito Isabel, Chaumeil Philippe, Dupuy Jean-William, Lomenech Anne Marie, Barré Aurélien, Gion Jean-Marc, Plomion Christophe. 2011. Interaction between environmental factors affects the accumulation of root proteins in hydroponically grown Eucalyptus globulus (Labill.). Plant Physiology and Biochemistry, 49 (1) : pp. 69-76.

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Eucalyptus globulus (Labill.) is used for pulp and paper production worldwide. In this report we studied changes in protein expression in one osmotically stressed elite clone widely used in industrial plantations in Spain. High molecular weight polyethylene glycol (PEG) was used as an osmoticum in the growing medium. Roots of rooted cuttings were sampled after 3 and 36 h of treatment.Water potential and abscissic acid content were measured in shoot and root apices to characterize the physiological states of the plants. Total soluble proteins from roots were extracted and separated using two-dimensional gel electrophoresis (2-DE). Gels were stained with Coomassie brillant blue for quantitative analysis of protein accumulation. From a total of 406 reproducible spots, 34 were found to be differentially expressed depending on treatment (osmotic versus control condition) and/or stress duration (3 h versus 36 h), and were further characterized by tandem mass spectrometry. Several proteins were reliably identified including adenosine kinase, actin, stress-related proteins as well as proteins associated to cellular processes, amongwhich some residents of the endoplasmic reticulum. This study constitutes the first investigation of the root proteome in this important forest tree genus. (Résumé d'auteur)

Mots-clés Agrovoc : Eucalyptus globulus, Racine, Protéine végétale, Identification, Électrophorèse, Réticulum endoplasmique, Stress osmotique, Physiologie végétale

Classification Agris : F60 - Plant physiology and biochemistry
F62 - Plant physiology - Growth and development
K10 - Forestry production

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Bedon Franck, INRA (FRA)
  • Majada Juan, SERIDA (ESP)
  • Feito Isabel, SERIDA (ESP)
  • Chaumeil Philippe, INRA (FRA)
  • Dupuy Jean-William, Université de Bordeaux (FRA)
  • Lomenech Anne Marie, Université de Bordeaux (FRA)
  • Barré Aurélien, Université de Bordeaux II (FRA)
  • Gion Jean-Marc, CIRAD-BIOS-UMR AGAP (FRA)
  • Plomion Christophe, INRA (FRA)

Source : Cirad - Agritrop (

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