Identification and analysis of polymorphisms in the promoter region of the gene DREB1A from contrasting haplotypes of Coffea canephora

Alves Gabriel Sergio Costa, Freire Luciana Pereira, Vieira Natalia Gomes, Marraccini Pierre, Paiva L.V., Andrade Alan Carvalho. 2013. Identification and analysis of polymorphisms in the promoter region of the gene DREB1A from contrasting haplotypes of Coffea canephora. In : Proceedings of the 24th International Conference on Coffee Science, San José, Costa Rica, 12th-16th November 2012 = Actes du 24ème Colloque scientifique international sur le café, San José, Costa Rica, 12-16 novembre 2012. ASIC. Paris : ASIC, 1424-1428. ISBN 978-2-900212-23-3 International Conference on Coffee Science. 24, San José, Costa Rica, 12 Novembre 2012/16 Novembre 2012.

Résumé : The aim of the present study was to identify and analyze the polymorphisms within the promoter region of the gene DREB1A in two clones of C. canephora, clones 14 (drought tolerant) and 22 (drought susceptible), but also in other accessions of Coffea genus (C. eugenioides and C. arabica). This allowed the identification of cis-regulatory elements and also of polymorphisms in the promoter region of the gene DREB1A. For example, the presence of cis-regulatory elements from transcription factors belonging to ABA-dependent pathway suggested the possibility of cross-talk between the two networks (ABA-independent and -dependent) response upon the regulation process of the DREB1A gene. The polymorphisms permitted the identification of different haplotypes among those genotypes and the allelic distribution of these loci. These polymorphisms may indicate the involvement of different haplotypes in the genetic control of drought tolerance.

Classification Agris : F30 - Génétique et amélioration des plantes
H50 - Troubles divers des plantes
F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Alves Gabriel Sergio Costa, EMBRAPA (BRA)
• Freire Luciana Pereira, EMBRAPA (BRA)
• Vieira Natalia Gomes, EMBRAPA (BRA)
• Marraccini Pierre, CIRAD-BIOS-UMR AGAP (BRA) ORCID: 0000-0001-7637-6811
• Paiva L.V., UFLA (BRA)
• Andrade Alan Carvalho, EMBRAPA (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/569402/)

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