New identification tools for Culicoides (Diptera: Ceratopogonidae") biting midge vectors of bluetongue virus: interactive identification key for female Culicoides of the Palaearctic region and quantitative-PCR assay for the Obsoletus complex

Mathieu Bruno, Cetre-Sossah Catherine, Garros Claire, Chavernac David, Balenghien Thomas, Vignes-Lebbe Régine, Riviere-Ung Visotheary, Candolfi Ermanno, Delecolle Jean Claude. 2010. New identification tools for Culicoides (Diptera: Ceratopogonidae") biting midge vectors of bluetongue virus: interactive identification key for female Culicoides of the Palaearctic region and quantitative-PCR assay for the Obsoletus complex. In : Proceedings of the 17th European Society for Vector Ecology Conference, 13th - 17th September 2010 (ESOVE 2010), Wroclaw, Poland : Conference Programme and abstract book. Eds. Katarzyna Rydzanicz, Elzbieta Lonc. s.l. : s.n., Résumé, 60. ISBN 978-83-60425-53-4 European Society for Vector Ecology Conference. 17, Wroclaw, Pologne, 13 Septembre 2010/17 Septembre 2010.

Résumé : ln 2006, bluetongue virus (BTV) outbreaks appeared surprisingly in northern Europe and widely affected most European countries. Correct identification of Culicoides species, is a key component of BTV vector studies. BTV epidemiology in Europe remains complicated in particular with the sympatric distribution of two sibling species, C. obsoletus and C. scoticus, which are suspected to be BTV vectors and may differ in their vector competence. ;,Our objectives were to develop new morphological and molecular identification tools for Culicoides. A cornputer-based system Xper2 was used to develop an Interactive Identification Key (IIKC) for female Culicoides from the West Palearctic region on morphological characters. Based on molecular data, 'a quantitative PCR assay has been designed to estimate the abundance of C. obsoletus and C. scoticus in samples. The current version ofIIKC includes 108taxa, 61 descriptors and 837 pictures and schemes. IIKC is a powerful tool to routinely identify Culicoides species and to train young specialized taxonomists. Specificity and sensitivity of the qPCR assay have been evaluated with 54 species and 9 samples of mixed C. obsoletusl scoticus specimens (0/100, 1/99, 5/95, 25/75, 50/50, 75/25, 95/5, 99/1 and 100/0). The development of new identification tools for arthropod vectors will help seientists in differentiating species and therefore give better insights into distribution, bionomics and vector control of these species.

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux

Auteurs et affiliations

• Mathieu Bruno, Faculté de médecine de Strasbourg (FRA)
• Cetre-Sossah Catherine, CIRAD-BIOS-UMR CMAEE (FRA)
• Garros Claire, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0003-4378-5031
• Chavernac David, CIRAD-BIOS-UMR CMAEE (FRA)
• Balenghien Thomas, CIRAD-BIOS-UMR CMAEE (FRA)
• Vignes-Lebbe Régine, Université Pierre et Marie Curie (FRA)
• Riviere-Ung Visotheary, CNRS (FRA)
• Candolfi Ermanno, Université de Strasbourg (FRA)
• Delecolle Jean Claude, Faculté de médecine de Strasbourg (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/569467/)

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