Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl- accumulation behaviour

Podda Alessandra, Checcucci Giovanni, Mouhaya Wafa, Centeno Delphine, Rofidal Valérie, Del Carratore Renata, Luro François, Morillon Raphaël, Ollitrault Patrick, Maserti Bianca Elena. 2013. Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl- accumulation behaviour. Journal of Plant Physiology, 170 (12) : pp. 1101-1112.

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : To understand the genotypic variation of citrus to mild salt stress, a proteomic approach has been carried out in parallel on two citrus genotypes ('Cleopatra' and 'Willow leaf' mandarins), which differ for Na+ and Cl? accumulation, and their cognate autotetraploids (4×). Using two-dimensional electrophoresis approximately 910 protein spots were reproducibly detected in control and salt-stressed leaves of all genotypes. Among them, 44 protein spots showing significant variations at least in one genotype were subjected to mass spectrometry analysis for identification. Salt-responsive proteins were involved in several functions, including photosynthetic processes, ROS scavenging, stress defence, and signalling. Genotype factors affect the salt-responsive pattern, especially that of carbon metabolism. The no ion accumulator 'Cleopatra' mandarin genotype showed the highest number of salt-responsive proteins, and up-regulation of Calvin cycle-related proteins. Conversely the ion accumulator 'Willow leaf' mandarin showed high levels of several photorespiration-related enzymes. A common set of proteins (twelve spots) displayed higher levels in salt-stressed leaves of 2× and 4× 'Cleopatra' and 4× 'Willow leaf' mandarin. Interestingly, antioxidant enzymes and heat shock proteins showed higher constitutive levels in 4× 'Cleopatra' mandarin and 4× 'Willow leaf' mandarin compared with the cognate 2× genotype. This work provides for the first time information on the effect of 8 weeks of salt stress on citrus genotypes contrasting for ion accumulation and their cognate autotetraploids. Results underline that genetic factors have a predominant effect on the salt response, although a common stress response independent from genotype was also found. (Résumé d'auteur)

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry
H50 - Miscellaneous plant disorders

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Podda Alessandra, CNR (ITA)
  • Checcucci Giovanni, CNR (ITA)
  • Mouhaya Wafa
  • Centeno Delphine, INRA (FRA)
  • Rofidal Valérie, INRA (FRA)
  • Del Carratore Renata, CNR (ITA)
  • Luro François, INRA (FRA)
  • Morillon Raphaël, CIRAD-BIOS-UMR AGAP (ESP)
  • Ollitrault Patrick, CIRAD-BIOS-UMR AGAP (ESP) ORCID: 0000-0002-9456-5517
  • Maserti Bianca Elena, CNR (ITA)

Source : Cirad - Agritrop (

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