Biased mutational pattern and quasispecies hypothesis in H5N1 virus

Gutiérrez Ramona Alikiiteaga, Viari Alain, Godelle Bernard, Frutos Roger, Buchy Philippe. 2013. Biased mutational pattern and quasispecies hypothesis in H5N1 virus. Infection, Genetics and Evolution, 15, spec. : pp. 69-76. International Giardia and Cryptosporidium Conference. 4, Wellington, Afrique du Sud, 30 January 2012/3 February 2012.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.

Télécharger (292kB)

Quartile : Q2, Sujet : INFECTIOUS DISEASES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie

Abstract : Like other RNA viruses, influenza viruses are subject to high mutation rates. Carrying segmented RNA genomes, their genetic variability is even higher. We aimed at analyzing the mutational events occurring during the infection of chickens by the Highly Pathogenic Avian Influenza (HPAI) H5N1 virus. We therefore studied the different sequences of two surface proteins, hemagglutinin (HA) and neuraminidase (NA), as well as two internal proteins, PB2 and NS. Three organs (lung, spleen, brain) were obtained from a chicken, experimentally infected with a lethal dose of HPAI H5N1 virus. Cloning these PCR fragments enabled us to investigate the mutations undergone by the virus after several replicative cycles. The first outcome is the presence of a strong mutational bias, resembling host-driven ADAR1 adenosine deamination, which is responsible for 81% of all mutations. Whereas the frequency of RNA dependent RNA polymerase-related mutations is compatible with the survival of the virus, the ADAR1-like activity usually strongly increases the mutation frequency into a level of "error catastrophe" in theory incompatible with virus survival. Nevertheless, the virus was successfully infective. HPAI H5N1 virus displayed traits in agreement with the quasispecies theory. The role of this quasispecies structure in successful infection and the superposition with the ADAR1-like response is discussed. (Résumé d'auteur)

Mots-clés Agrovoc : Influenzavirus aviaire, Mutation, Volaille, Génétique des populations, Infection, Polymorphisme génétique, Modèle

Mots-clés géographiques Agrovoc : Cambodge, France

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Gutiérrez Ramona Alikiiteaga, Réseau International des Instituts Pasteur (KHM)
  • Viari Alain, INRIA (FRA)
  • Godelle Bernard, UM2 (FRA)
  • Buchy Philippe, Institut Pasteur du Cambodge (KHM)

Source : Cirad - Agritrop (

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-01-17 ]