Whole genome mapping and re-organization of the nuclear and mitochondrial genomes of Babesia microti isolates

Cornillot Emmanuel, Dassouli Amina, Garg Aprajita, Pachikara Niseema, Randazzo Sylvie, Depoix Delphine, Carcy Bernard, Delbecq Stéphane, Frutos Roger, Silva Joana C., Sutton Richard, Krause Peter J., Mamoun Choukri Ben. 2013. Whole genome mapping and re-organization of the nuclear and mitochondrial genomes of Babesia microti isolates. PloS One, 8 (9):e72657, 12 p.

Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Babesia microti is the primary causative agent of human babesiosis, an emerging pathogen that causes a malaria-like illness with possible fatal outcome in immunocompromised patients. The genome sequence of the B. microti R1 strain was reported in 2012 and revealed a distinct evolutionary path for this pathogen relative to that of other apicomplexa. Lacking from the first genome assembly and initial molecular analyses was information about the terminal ends of each chromosome, and both the exact number of chromosomes in the nuclear genome and the organization of the mitochondrial genome remained ambiguous. We have now performed various molecular analyses to characterize the nuclear and mitochondrial genomes of the B. microti R1 and Gray strains and generated high-resolution Whole Genome maps. These analyses show that the genome of B. microti consists of four nuclear chromosomes and a linear mitochondrial genome present in four different structural types. Furthermore, Whole Genome mapping allowed resolution of the chromosomal ends, identification of areas of misassembly in the R1 genome, and genomic differences between the R1 and Gray strains, which occur primarily in the telomeric regions. These studies set the stage for a better understanding of the evolution and diversity of this important human pathogen.

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Cornillot Emmanuel, Institut de Biologie Computationnelle (FRA)
• Dassouli Amina, Yale University (USA)
• Garg Aprajita, Yale University (USA)
• Pachikara Niseema, Yale University (USA)
• Randazzo Sylvie, UM1 (FRA)
• Depoix Delphine, UM1 (FRA)
• Carcy Bernard, UM1 (FRA)
• Delbecq Stéphane, UM1 (FRA)
• Frutos Roger, CIRAD-BIOS-UMR INTERTRYP (FRA)
• Silva Joana C., University of Maryland (USA)
• Sutton Richard, Yale University (USA)
• Krause Peter J., Yale University (USA)
• Mamoun Choukri Ben, Yale University (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/571433/)

Métriques

[ Page générée et mise en cache le 2024-04-02 ]