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The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness

Gebelin Virginie, Leclercq Julie, Kuswanhadi, Argout Xavier, Chaidamsari Tetty, Hu Songnian, Tang Chaorong, Sarah Gautier, Yang Meng, Montoro Pascal. 2013. The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness. Tree Physiology, 33 (10) : 1084-1098.

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Quartile : Q1, Sujet : FORESTRY

Résumé : Natural rubber is harvested by tapping Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. Harvesting stress can lead to tapping panel dryness (TPD). MicroRNAs (miRNAs) are induced by abiotic stress and regulate gene expression by targeting the cleavage or translational inhibition of target messenger RNAs. This study set out to sequence miRNAs expressed in latex cells and to identify TPD-related putative targets. Deep sequencing of small RNAs was carried out on latex from trees affected by TPD using Solexa technology. The most abundant small RNA class size was 21 nucleotides for TPD trees compared with 24 nucleotides in healthy trees. By combining the LeARN pipeline, data from the Plant MicroRNA database and Hevea EST sequences, we identified 19 additional conserved and four putative species-specific miRNA families not found in previous studies on rubber. The relative transcript abundance of the Hbpre-MIR159b gene increased with TPD. This study revealed a small RNA-specific signature of TPD-affected trees. Both RNA degradation and a shift in miRNA biogenesis are suggested to explain the general decline in small RNAs and, particularly, in miRNAs. (Résumé d'auteur)

Mots-clés Agrovoc : Hevea brasiliensis, Expression des gènes, Régulation physiologique, ARN, Séquence nucléotidique, Latex, Stress, Récolte, Oxydation

Mots-clés complémentaires : Séquencage

Classification Agris : F30 - Génétique et amélioration des plantes
F01 - Culture des plantes
F62 - Physiologie végétale : croissance et développement

Axe stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Gebelin Virginie
  • Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
  • Kuswanhadi, Indonesian Rubber Research Institute (IDN)
  • Argout Xavier, CIRAD-BIOS-UMR AGAP (FRA)
  • Chaidamsari Tetty, IBRIEC (IDN)
  • Hu Songnian, Chinese Academy of Sciences (CHN)
  • Tang Chaorong, CATAS (CHN)
  • Sarah Gautier, INRA (FRA)
  • Yang Meng, Chinese Academy of Sciences (CHN)
  • Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad - Agritrop

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