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Identification of QTLs controlling yield traits in sweet sorghum using genotyping by sequencing

Pereira Guilherme S., Souza Vander Fillipe, Parrella Rafael Augusto C, Damasceno Cynthia Maria B, Simeone Maria Lucia F., Schaffert Robert E., Braconnier Serge, Pastina M.M., Garcia Antonio Augusto Franco, Magalhaes Jurandir V.. 2013. Identification of QTLs controlling yield traits in sweet sorghum using genotyping by sequencing. In : Resumos do 59° Congresso Brasileiro de Genética,16 a 19 de Setembro de 2013, Águas de Lindóia, Brasil. SBG. s.l. : s.n., Résumé, p. 60. ISBN 978-85-89109-06-2 Congresso Brasileiro de Genética. 59, Aguas de Lindoia, Brésil, 16 September 2013/19 September 2013.

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Abstract : Sweet sorghum, a C4 tropical grass, is becoming an important bioenergy crop. Characterizing quantitative trait loci (QTLs) underlying bioenergy-related traits can lead to the identification of target regions for molecular breeding programs. These QTLs may be conserved in related species such as sugarcane, expanding their potential usefulness for bioenergy production. In this study, 223 recombinant inbred lines (RILs) derived from the sweet sorghum parents, Brandes and Wray, were genotyped by sequencing (GBS). A lattice experiment with three replicates was established in two years for evaluating four important yield traits: plant height (in m), plot weight (in kg), sucrose content (or Pol, in %), and volume of hydrated alcohol (in L). Phenotypic analysis and QTL scanning were conducted using mixed and general linear models approaches, in the respective softwares GenStat and TASSEL. GBS provided wide genome coverage, with ~1100 single-nucleotide polymorphisms (SNPs) per chromosome. Variance-covariance matrices for genetic and residual effects were appropriately fitted, allowing heterogeneous variances for harvest years. Major QTLs were co-located for plant height and plot weight on chromosome 10, and for Pol and hydrated alcohol on chromosome 3, explaining 20.7%, 14.0%, 23.1% and 23.8% of the phenotypic variance, respectively. Suggestive QTLs were additionally detected on others chromosomes for all traits. The QTLs identified here will be further investigated for possible use in marker-assisted selection targeting biofuel production in sweet sorghum. Financial Support: FAPESP, CNPq, Embrapa, and Seventh Framework Programme (FP7). (Résumé d'auteur)

Classification Agris : F30 - Plant genetics and breeding
P06 - Renewable energy resources
F01 - Crop husbandry

Auteurs et affiliations

  • Pereira Guilherme S., Universidade de São Paulo (BRA)
  • Souza Vander Fillipe, Federal University of São João Del-Rei (BRA)
  • Parrella Rafael Augusto C, EMBRAPA (BRA)
  • Damasceno Cynthia Maria B, EMBRAPA (BRA)
  • Simeone Maria Lucia F., EMBRAPA (BRA)
  • Schaffert Robert E., EMBRAPA (BRA)
  • Braconnier Serge, CIRAD-BIOS-UMR AGAP (FRA)
  • Pastina M.M., EMBRAPA (BRA)
  • Garcia Antonio Augusto Franco, Universidade de São Paulo (BRA)
  • Magalhaes Jurandir V., EMBRAPA (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/571644/)

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