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Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads

Darrasse Armelle, Carrère Sébastien, Barbe Valérie, Boureau Tristan, Arrieta-Ortiz Mario L., Bonneau Sophie, Briand Martial, Brin Christelle, Cociancich Stéphane, Durand Karine, Fouteau Stéphanie, Gagnevin Lionel, Guérin Fabien, Guy Endrick, Indiana Arnaud, Koebnik Ralf, Lauber Emmanuelle, Munoz Alejandra, Noel Laurent D., Pieretti Isabelle, Poussier Stéphane, Pruvost Olivier, Robène-Soustrade Isabelle, Rott Philippe, Royer Monique, Serres-Giardi Laurana, Szurek Boris, Van Sluys Marie-Anne, Verdier Valérie, Vernière Christian, Arlat Mathieu, Manceau Charles, Jacques Marie Agnès. 2013. Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads. BMC Genomics, 14 (761), 30 p.

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Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q1, Sujet : GENETICS & HEREDITY

Abstract : Background Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences. Results Comparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations. Conclusions This work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness. (Résumé d'auteur)

Mots-clés Agrovoc : Xanthomonas, Génie génétique, Génome, Séquence nucléotidique, Effecteur moléculaire, Pseudogène, Adaptation physiologique, Phaseolus vulgaris

Mots-clés complémentaires : Xanthomonas fuscans, Séquencage

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Darrasse Armelle, INRA (FRA)
  • Carrère Sébastien, INRA (FRA)
  • Barbe Valérie, Centre national de séquençage (FRA)
  • Boureau Tristan, Université d'Angers (FRA)
  • Arrieta-Ortiz Mario L., INRA (FRA)
  • Bonneau Sophie, Agrocampus Ouest (FRA)
  • Briand Martial, INRA (FRA)
  • Brin Christelle, INRA (FRA)
  • Cociancich Stéphane, CIRAD-BIOS-UMR BGPI (FRA)
  • Durand Karine, INRA (FRA)
  • Fouteau Stéphanie, CEA (FRA)
  • Gagnevin Lionel, CIRAD-BIOS-UMR PVBMT (REU)
  • Guérin Fabien, Université de la Réunion (REU)
  • Guy Endrick, INRA (FRA)
  • Indiana Arnaud, Agrocampus Ouest (FRA)
  • Koebnik Ralf, IRD (FRA)
  • Lauber Emmanuelle, INRA (FRA)
  • Munoz Alejandra, Universidad de los Andes (COL)
  • Noel Laurent D., INRA (FRA)
  • Pieretti Isabelle, CIRAD-BIOS-UMR BGPI (FRA)
  • Poussier Stéphane, Université de la Réunion (REU)
  • Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)
  • Robène-Soustrade Isabelle, CIRAD-BIOS-UMR PVBMT (REU)
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159
  • Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
  • Serres-Giardi Laurana
  • Szurek Boris, IRD (FRA)
  • Van Sluys Marie-Anne, Universidade de São Paulo (BRA)
  • Verdier Valérie, IRD (FRA)
  • Vernière Christian, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-2312-2073
  • Arlat Mathieu, Université Toulouse III Paul Sabatier (FRA)
  • Manceau Charles, INRA (FRA)
  • Jacques Marie Agnès, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/572053/)

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