Genome wide profiling of Azospirillum lipoferum 4B gene expression during interaction with rice roots

Drogue Benoit, Sanguin Hervé, Borland Stéphanie, Prigent-Combaret Claire, Wisniewski-Dyé Florence. 2014. Genome wide profiling of Azospirillum lipoferum 4B gene expression during interaction with rice roots. FEMS Microbiology, Ecology, 87 (2) : 543-555.

Quartile : Q2, Sujet : MICROBIOLOGY

Résumé : Azospirillum-plant cooperation has been mainly studied from an agronomic point of view leading to a wide description of mechanisms implicated in plant growth-promoting effects. However, little is known about genetic determinants implicated in bacterial adaptation to the host plant during the transition from free-living to root-associated lifestyles. This study aims at characterizing global gene expression of Azospirillum lipoferum 4B following a 7-day-old interaction with two cultivars of Oryza sativa L. japonica (cv. Cigalon from which it was originally isolated, and cv. Nipponbare). The analysis was done on a whole genome expression array with RNA samples obtained from planktonic cells, sessile cells, and root-adhering cells. Root-associated Azospirillum cells grow in an active sessile-like state and gene expression is tightly adjusted to the host plant. Adaptation to rice seems to involve genes related to reactive oxygen species (ROS) detoxification and multidrug efflux, as well as complex regulatory networks. As revealed by the induction of genes encoding transposases, interaction with root may drive bacterial genome rearrangements. Several genes related to ABC transporters and ROS detoxification display cultivar-specific expression profiles, suggesting host specific adaptation and raising the question of A. lipoferum 4B/rice cv. Cigalon co-adaptation.

Mots-clés Agrovoc : Oryza sativa, Azospirillum, Rhizobactérie, interactions biologiques, plante hôte, génome, expression des gènes, transcription, adaptation, système racinaire, développement biologique

Mots-clés complémentaires : Azospirillum lipoferum, Oryza sativa japonica

Classification Agris : F62 - Physiologie végétale - Croissance et développement
P34 - Biologie du sol
F01 - Culture des plantes

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Drogue Benoit, CNRS (FRA)
• Sanguin Hervé, CIRAD-BIOS-UMR LSTM (FRA)
• Borland Stéphanie, CNRS (FRA)
• Prigent-Combaret Claire, CNRS (FRA)
• Wisniewski-Dyé Florence, CNRS (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/572513/)

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