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The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants

Porcel Betina M., Denoeud France, Opperdoes Fred, Noël Benjamin, Madoui Mohammed-Amine, Hammarton Tansy, Field Mark, Da Silva Corinne, Couloux Arnaud, Poulain Julie, Katinka Michael, Jabbari Kamel, Aury Jean-Marc, Campbell David A., Cintron Roxana, Dickens Nicolas, Docampo Roberto, Sturm Nancy R., Koumandou Lila, Fabre Sandrine, Flegontov Pavel, Lukes Julius, Michaeli Shula, Mottram Jeremy, Szoor Balazs, Zilberstein Dan, Bringaud Frédéric, Wincker Patrick, Dollet Michel. 2014. The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants. PLoS Genetics, 10 (2):e1004007, 24 p.

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Quartile : Outlier, Sujet : GENETICS & HEREDITY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie

Abstract : Members of the family Trypanosomatidae infect many organisms, including animals, plants and humans. Plant-infecting trypanosomes are grouped under the single genus Phytomonas, failing to reflect the wide biological and pathological diversity of these protists. While some Phytomonas spp. multiply in the latex of plants, or in fruit or seeds without apparent pathogenicity, others colonize the phloem sap and afflict plants of substantial economic value, including the coffee tree, coconut and oil palms. Plant trypanosomes have not been studied extensively at the genome level, a major gap in understanding and controlling pathogenesis. We describe the genome sequences of two plant trypanosomatids, one pathogenic isolate from a Guianan coconut and one non-symptomatic isolate from Euphorbia collected in France. Although these parasites have extremely distinct pathogenic impacts, very few genes are unique to either, with the vast majority of genes shared by both isolates. Significantly, both Phytomonas spp. genomes consist essentially of single copy genes for the bulk of their metabolic enzymes, whereas other trypanosomatids e.g. Leishmania and Trypanosoma possess multiple paralogous genes or families. Indeed, comparison with other trypanosomatid genomes revealed a highly streamlined genome, encoding for a minimized metabolic system while conserving the major pathways, and with retention of a full complement of endomembrane organelles, but with no evidence for functional complexity. Identification of the metabolic genes of Phytomonas provides opportunities for establishing in vitro culturing of these fastidious parasites and new tools for the control of agricultural plant disease. (Résumé d'auteur)

Mots-clés Agrovoc : Phytomonas, Cocos nucifera, Euphorbia, Génome, Séquence nucléotidique, Agent pathogène, Phloème, Identification, Culture in vitro, Contrôle de maladies, Transcription d'ADN, ADN, Phylogénie, Trypanosoma brucei, Trypanosoma cruzi, Trypanosoma, Maladie de l'homme, Maladie des plantes

Mots-clés géographiques Agrovoc : Guyane française, France

Mots-clés complémentaires : Séquencage

Classification Agris : H20 - Plant diseases
L73 - Animal diseases
000 - Other themes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Porcel Betina M., CEA (FRA)
  • Denoeud France, CEA (FRA)
  • Opperdoes Fred, UCL (BEL)
  • Noël Benjamin, CEA (FRA)
  • Madoui Mohammed-Amine, CEA (FRA)
  • Hammarton Tansy, University of Glasgow (GBR)
  • Field Mark, University of Cambridge (GBR)
  • Da Silva Corinne, Institut de génomique (FRA)
  • Couloux Arnaud, Centre national de séquençage (FRA)
  • Poulain Julie, Institut de génomique (FRA)
  • Katinka Michael, CEA (FRA)
  • Jabbari Kamel, CEA (FRA)
  • Aury Jean-Marc, Institut de génomique (FRA)
  • Campbell David A., UCLA (USA)
  • Cintron Roxana, University of Georgia (USA)
  • Dickens Nicolas, University of Glasgow (GBR)
  • Docampo Roberto, University of Georgia (USA)
  • Sturm Nancy R., UCLA (USA)
  • Koumandou Lila, Academy of Athens (GRC)
  • Fabre Sandrine, CIRAD-BIOS-UMR RPB (FRA)
  • Flegontov Pavel, University of South Bohemia (CZE)
  • Lukes Julius, University of South Bohemia (CZE)
  • Michaeli Shula, Bar-Ilan University (ISR)
  • Mottram Jeremy, IEM (FRA)
  • Szoor Balazs, University of Edinburgh (GBR)
  • Zilberstein Dan, Technion (ISR)
  • Bringaud Frédéric, Université de Bordeaux (FRA)
  • Wincker Patrick, Institut de génomique (FRA)
  • Dollet Michel, CIRAD-BIOS-UMR RPB (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/572520/)

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