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Functional analysis of the durum wheat gene TdPIP2;1 and itspromoter region in response to abiotic stress in rice

Ayadi Malika, Mieulet Delphine, Fabre Denis, Verdeil Jean-Luc, Vernet Aurore, Guiderdoni Emmanuel, Masmoudi Khaled. 2014. Functional analysis of the durum wheat gene TdPIP2;1 and itspromoter region in response to abiotic stress in rice. Plant Physiology and Biochemistry, 79 : pp. 98-108.

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : In a previous work, we demonstrated that expression of TdPIP2;1 in Xenopus oocytes resulted in an increase in Pf compared to water injected oocytes. Phenotypic analyses of transgenic tobacco plants expressing TdPIP2;1 generated a tolerance phenotype towards drought and salinity stresses. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter region of the TdPIP2;1 gene. A 1060-bp genomic fragment upstream of the TdPIP2;1 translated sequence has been isolated, cloned, and designated as the proTdPIP2;1 promoter. Sequence analysis of proTdPIP2;1 revealed the presence of cis regulatory elements which could be required for abiotic stress responsiveness, for tissue-specific and vascular expression. The proTdPIP2;1 promoter was fused to the bglucuronidase (gusA) gene and the resulting construct was transferred into rice (cv. Nipponbare). Histochemical analysis of proTdPIP2;1::Gus in rice plants revealed that the GUS activity was observed in leaves, stems and roots of stably transformed rice T3 plants. Histological sections prepared revealed accumulation of GUS products in phloem, xylem and in some cells adjacent to xylem. The transcripts were up-regulated by dehydration. Transgenic rice plants overexpressing proTdPIP2;1 in fusion with TdPIP2;1, showed enhanced drought tolerance, while wild type plants were more sensitive and exhibited symptoms of wilting and chlorosis. These findings suggest that expression of the TdPIP2;1 gene regulated by its own promoter achieves enhanced drought tolerance in rice. (Résumé d'auteur)

Mots-clés Agrovoc : Oryza sativa, Plante transgénique, Résistance à la sécheresse, Expression des gènes, Transcription, Triticum turgidum, Gène, Stress, Transpiration

Mots-clés géographiques Agrovoc : Tunisie

Mots-clés complémentaires : Promoteur

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry
H50 - Miscellaneous plant disorders

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Ayadi Malika, Centre of Biotechnology of Sfax (TUN)
  • Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
  • Fabre Denis, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-6222-2587
  • Verdeil Jean-Luc, CIRAD-BIOS-UMR AGAP (FRA)
  • Vernet Aurore, CIRAD-BIOS-UMR AGAP (FRA)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Masmoudi Khaled, Centre of Biotechnology of Sfax (TUN)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/573137/)

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