Production of cecropin A antimicrobial peptide in rice seed endosperm

Mireia Bundó, Montesinos Laura, Izquierdo Esther, Campo Sonia, Mieulet Delphine, Guiderdoni Emmanuel, Rossignol Michel, Badosa Esther, Montesinos Emilio, San Segundo Blanca, Coca Maria. 2014. Production of cecropin A antimicrobial peptide in rice seed endosperm. BMC Plant Biology, 14 (102), 29 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Background Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against a broad spectrum of pathogens, thus having great biotechnological potential. Here, we report a system for producing bioactive cecropin A in rice seeds. Results Transgenic rice plants expressing a codon-optimized synthetic cecropin A gene drived by an endosperm-specific promoter, either the glutelin B1 or glutelin B4 promoter, were generated. The signal peptide sequence from either the glutelin B1 or the glutelin B4 were N-terminally fused to the coding sequence of the cecropin A. We also studied whether the presence of the KDEL endoplasmic reticulum retention signal at the C-terminal has an effect on cecropin A subcellular localization and accumulation. The transgenic rice plants showed stable transgene integration and inheritance. We show that cecropin A accumulates in protein storage bodies in the rice endosperm, particularly in type II protein bodies, supporting that the glutelin Nterminal signal peptides play a crucial role in directing the cecropin A to this organelle, independently of being tagged with the KDEL endoplasmic reticulum retention signal. The production of cecropin A in transgenic rice seeds did not affect seed viability or seedling growth. Furthermore, transgenic cecropin A seeds exhibited resistance to infection by fungal and bacterial pathogens (Fusarium verticillioides and Dickeya dadantii, respectively) indicating that the in planta-produced cecropin A is biologically active. Conclusions Rice seeds can sustain bioactive cecropin A production and accumulation in protein bodies. The system might benefit the production of this antimicrobial agent for subsequent applications in crop protection and food preservation. (Résumé d'auteur)

Mots-clés Agrovoc : Oryza sativa, Grain, Endosperme, Bactéricide, Biosynthèse, Peptide, Propriété antimicrobienne, Résistance génétique, Résistance aux organismes nuisibles, Enterobacteriaceae, Fusarium, Plante transgénique, Amélioration des plantes, Physiologie végétale, Protection des plantes, Préservation des aliments

Mots-clés complémentaires : Dickeya dadantii, Fusarium verticilliodes

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry
H01 - Protection of plants - General aspects
Q03 - Food contamination

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Mireia Bundó, CRAG (ESP)
  • Montesinos Laura, Universidad de Girona (ESP)
  • Izquierdo Esther, Montpellier SupAgro (FRA)
  • Campo Sonia, CRAG (ESP)
  • Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Rossignol Michel, INRA (FRA)
  • Badosa Esther, Universidad de Girona (ESP)
  • Montesinos Emilio, Universidad de Girona (ESP)
  • San Segundo Blanca, CRAG (ESP)
  • Coca Maria, CRAG (ESP)

Source : Cirad - Agritrop (

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