Isolated cell walls exhibit cation binding properties distinct from those of plant roots

Guigues Stéphanie, Bravin Matthieu, Garnier Cédric, Masion Armand, Doelsch Emmanuel. 2014. Isolated cell walls exhibit cation binding properties distinct from those of plant roots. Plant and Soil, 381 (1-2) : pp. 367-379.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : AGRONOMY / Quartile : Q1, Sujet : SOIL SCIENCE / Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : The principal contributor to the cation binding properties of roots is currently considered to be the cell wall or, alternatively, the plasma membrane. The aim of this study was to highlight their respective contributions in the binding properties. Methods Cell walls of a dicotyledon (Solanum lycopersicum L.) and monocotyledon (Triticum aestivum L.) were isolated from roots and their binding properties were compared to those of their respective roots. Cell wall and root binding capacities were evaluated by potentiometric titrations and cation exchange capacity measurements, while their biochemical composition was analyzed by 13C-NMR spectroscopy. Results The lower binding capacity of isolated cell walls compared to roots revealed that cell plasma membranes had a higher binding site density than cell walls. The significant decrease in some NMR signals, i.e. carbonyl C, N alkyl/methoxyl C and alkyl C regions, suggested that carboxyl, amine and phosphate binding sites, borne by proteins and phospholipid plasma membranes, contribute to the binding capacity. Conclusions Cell walls and plasma membranes were found to be jointly involved in root binding properties and their respective contributions seemed vary between plants. (Résumé d'auteur)

Mots-clés géographiques Agrovoc : Réunion

Classification Agris : F62 - Plant physiology - Growth and development
P33 - Soil chemistry and physics
H50 - Miscellaneous plant disorders

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Guigues Stéphanie, CIRAD-PERSYST-UPR Recyclage et risque (FRA)
  • Bravin Matthieu, CIRAD-PERSYST-UPR Recyclage et risque (REU) ORCID: 0000-0002-1436-7837
  • Garnier Cédric, Université du Sud Toulon-Var (FRA)
  • Masion Armand, Université d'Aix-Marseille (FRA)
  • Doelsch Emmanuel, CIRAD-PERSYST-UPR Recyclage et risque (FRA) ORCID: 0000-0002-7478-4296

Source : Cirad - Agritrop (

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