Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis

Piyatrakul Piyanuch, Yang Meng, Putranto Riza Arief, Pirrello Julien, Dessailly Florence, Hu Songnian, Summo Marilyne, Theeravatanasuk Kannikar, Leclercq Julie, Kuswanhadi, Montoro Pascal. 2014. Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis. PloS One, 9 (6):e99367, 20 p.

https://doi.org/10.1371/journal.pone.0099367

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Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : The AP2/ERF superfamily encodes transcription factors that play a key role in plant development and responses to abiotic and biotic stress. In Hevea brasiliensis, ERF genes have been identified by RNA sequencing. This study set out to validate the number of HbERF genes, and identify ERF genes involved in the regulation of latex cell metabolism. A comprehensive Hevea transcriptome was improved using additional RNA reads from reproductive tissues. Newly assembled contigs were annotated in the Gene Ontology database and were assigned to 3 main categories. The AP2/ERF superfamily is the third most represented compared with other transcription factor families. A comparison with genomic scaffolds led to an estimation of 114 AP2/ERF genes and 1 soloist in Hevea brasiliensis. Based on a phylogenetic analysis, functions were predicted for 26 HbERF genes. A relative transcript abundance analysis was performed by real-time RT-PCR in various tissues. Transcripts of ERFs from group I and VIII were very abundant in all tissues while those of group VII were highly accumulated in latex cells. Seven of the thirty-five ERF expression marker genes were highly expressed in latex. Subcellular localization and transactivation analyses suggested that HbERF-VII candidate genes encoded functional transcription factors.

Mots-clés Agrovoc : Hevea brasiliensis, latex, métabolisme, gène, identification, séquence nucléotidique, expression des gènes, transcription

Mots-clés géographiques Agrovoc : Indonésie, Thaïlande

Mots-clés complémentaires : Séquencage

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

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