Microsatellites Cross-Species Amplification across Some African Cichlids

Bezault Etienne, Rognon François, Gharbi Karim, Baroiller Jean-François, Chevassus Bernard. 2012. Microsatellites Cross-Species Amplification across Some African Cichlids. International Journal of Evolutionary Biology, 2012 (ID 870935), 7 p.

Résumé : The transfer of the genomic resources developed in the Nile tilapia, Oreochromis niloticus, to other Tilapiines sensu lato and African cichlid would provide new possibilities to study this amazing group from genetics, ecology, evolution, aquaculture, and conservation point of view. We tested the cross-species amplification of 32 O. niloticus microsatellite markers in a panel of 15 species from 5 different African cichlid tribes: Oreochromines (Oreochromis, Sarotherodon), Boreotilapiines (Tilapia), Chromidotilapines, Hemichromines, and Haplochromines. Amplification was successfully observed for 29 markers (91%), with a frequency of polymorphic (P95) loci per species around 70%. The mean number of alleles per locus and species was 3.2 but varied from 3.7 within Oreochromis species to 1.6 within the nontilapia species. The high level of cross-species amplification and polymorphism of the microsatellite markers tested in this study provides powerful tools for a wide range of molecular genetic studies within tilapia species as well as for other African cichlids.

Mots-clés Agrovoc : Cichlidae, Oreochromis niloticus, tilapia (genre), génie génétique, microsatellite, marqueur génétique, polymorphisme génétique, aquaculture

Mots-clés géographiques Agrovoc : Afrique

Classification Agris : M12 - Production de l'aquaculture
L10 - Génétique et amélioration des animaux

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Bezault Etienne, INRA (FRA)
• Rognon François, INRA (FRA)
• Gharbi Karim, INRA (FRA)
• Baroiller Jean-François, CIRAD-PERSYST-UMR INTREPID (FRA) ORCID: 0000-0002-0992-1725
• Chevassus Bernard, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/574030/)

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