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Genetic structure of Bemisia tabaci Med populations fromhome-range countries, inferred by nuclear and cytoplasmic markers: impact on the distribution of the insecticide resistance genes

Gauthier Nathalie, Clouet Cécile, Perrakis Andreas, Kapantaidaki Despoina, Peterschmitt Michel, Tsagkarakou A.. 2014. Genetic structure of Bemisia tabaci Med populations fromhome-range countries, inferred by nuclear and cytoplasmic markers: impact on the distribution of the insecticide resistance genes. Pest Management Science, 70 (10) : pp. 1477-1491.

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Quartile : Q1, Sujet : ENTOMOLOGY / Quartile : Q1, Sujet : AGRONOMY

Abstract : BACKGROUND Insecticide resistance management in Bemisia tabaci is one of the main issues facing agricultural production today. An extensive survey was undertaken in five Mediterranean countries to examine the resistance status of Med B. tabaci species in its range of geographic origin and the relationship between population genetic structure and the distribution of resistance genes. The investigation combined molecular diagnostic tests, sequence and microsatellite polymorphism studies and monitoring of endosymbionts. RESULTS: High frequencies of pyrethroid (L925I and T929V, VGSC gene) and organophosphate (F331W, ace1 gene) resistance mutationswere found in France, Spain and Greece, but not in Morocco or Tunisia. Sequence analyses of the COI gene delineated two closely relatedmitochondrial groups (Q1 andQ2), whichwere found either sympatrically (Spain) or separately (France).Only Q1 was observed in Greece, Morocco and Tunisia. Bayesian analyses based on microsatellite loci revealed three geographically delineated genetic groups (France, Spain, Morocco/Greece/Tunisia) and high levels of genetic differentiation even between neighbouring samples. Evidence was also found for hybridisation and asymmetrical gene flow between Q1 and Q2. CONCLUSIONS: Med B. tabaci is more diverse and structured than reported so far. On a large geographic scale, resistance is affected by population genetic structure, whereas on a local scale, agricultural practices appear to play a major role. (Résumé d'auteur)

Mots-clés Agrovoc : Bemisia tabaci, Résistance aux pesticides, Insecticide, Génétique des populations, Marqueur génétique, Mutation, Flux de gènes, Mitochondrie, Cytoplasme, Plante de culture, Solanaceae, Cucurbitaceae, Plante ornementale, Lantana camara

Mots-clés géographiques Agrovoc : Espagne, Tunisie, Maroc, France, Grèce, Région méditerranéenne

Mots-clés complémentaires : Endosymbiote

Classification Agris : H10 - Pests of plants

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Gauthier Nathalie, IRD (FRA)
  • Clouet Cécile, INRA (FRA)
  • Perrakis Andreas, Hellenic Agricultural Organization (GRC)
  • Kapantaidaki Despoina, University of Western Greece (GRC)
  • Peterschmitt Michel, CIRAD-BIOS-UMR BGPI (FRA)
  • Tsagkarakou A., Hellenic Agricultural Organization (GRC)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/574246/)

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