Molecular cloning and characterization of a geranyl diphosphate-specific aromatic prenyltransferase from Lemon

Munakata Ryosuke, Inoue Tsuyoshi, Koeduka Takao, Karamat Fazeelat, Olry Alexandre, Sugiyama Akifumi, Takanashi Kojiro, Dugrand Audray, Froelicher Yann, Tanaka Ryo, Uto Yoshihiro, Hori Hitoshi, Azuma Jun-Ichi, Hehn Alain, Bourgaud Frédéric, Yazaki Kazufumi. 2014. Molecular cloning and characterization of a geranyl diphosphate-specific aromatic prenyltransferase from Lemon. Plant Physiology, 166 (1) : pp. 80-90.

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Quartile : Outlier, Sujet : PLANT SCIENCES

Abstract : Prenyl residues confer divergent biological activities such as antipathogenic and antiherbivorous activities on phenolic compounds, including flavonoids, coumarins, and xanthones. To date, about 1,000 prenylated phenolics have been isolated, with these compounds containing various prenyl residues. However, all currently described plant prenyltransferases (PTs) have been shown specific for dimethylallyl diphosphate as the prenyl donor, while most of the complementary DNAs encoding these genes have been isolated from the Leguminosae. In this study, we describe the identification of a novel PT gene from lemon (Citrus limon), ClPT1, belonging to the homogentisate PT family. This gene encodes a PT that differs from other known PTs, including flavonoid-specific PTs, in polypeptide sequence. This membrane-bound enzyme was specific for geranyl diphosphate as the prenyl donor and coumarin as the prenyl acceptor. Moreover, the gene product was targeted to plastid in plant cells. To our knowledge, this is the novel aromatic PT specific to geranyl diphosphate from citrus species. (Résumé d'auteur)

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Munakata Ryosuke, Kyoto University (JPN)
  • Inoue Tsuyoshi, Kyoto University (JPN)
  • Koeduka Takao, Kyoto University (JPN)
  • Karamat Fazeelat, INRA (FRA)
  • Olry Alexandre, INRA (FRA)
  • Sugiyama Akifumi, Kyoto University (JPN)
  • Takanashi Kojiro, Kyoto University (JPN)
  • Dugrand Audray, INRA (FRA)
  • Froelicher Yann, CIRAD-BIOS-UMR AGAP (FRA)
  • Tanaka Ryo, University of Tokushima (JPN)
  • Uto Yoshihiro, University of Tokushima (JPN)
  • Hori Hitoshi, INRA (FRA)
  • Azuma Jun-Ichi, Kyoto University (JPN)
  • Hehn Alain, INRA (FRA)
  • Bourgaud Frédéric, INRA (FRA)
  • Yazaki Kazufumi, Kyoto University (JPN)

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