Formation of vacuolar tannin deposits in the chlorophyllous organs of Tracheophyta: from shuttles to accretions

Brillouet Jean-Marc, Romieu Charles, Lartaud Marc, Jublanc Elodie, Torregrosa Laurent, Cazevieille Chantal. 2014. Formation of vacuolar tannin deposits in the chlorophyllous organs of Tracheophyta: from shuttles to accretions. Protoplasma, 251 (6) : 1387-1393.

Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q3, Sujet : CELL BIOLOGY

Résumé : Most Tracheophyta synthesize-condensed tannins (also called proanthocyanidins), polymers of catechins, which appear in the vacuole as uniformly stained deposits-termed tannin accretions-lining the inner face of the tonoplast. A large body of evidence argues that tannins are formed in recently described thylakoid-derived organelles, the tannosomes, which are packed in membrane-bound shuttles (Brillouet et al. 2013); it has been suggested that shuttles agglomerate into tannin accretions. The aim of the study was to describe the ontogenesis of tannin accretions in members of the Tracheophyta. For this purpose, fresh specimens of young tissues from diverse Tracheophyta were cut, gently lacerated in paraformaldehyde, and examined using light, epifluorescence, confocal, and transmission electron microscopy. Fresh samples were also incubated with gelatin-Oregon Green, a fluorescent marker of condensed tannins. Our observations showed that vacuolar accretions (1→40 μm), that constitute the typical form of tannin storage in tannin-producing Tracheophyta, are formed by agglomeration (not fusion) of shuttles containing various proportions of chlorophylls and tannins.

Mots-clés géographiques Agrovoc : Languedoc-Roussillon, France

Classification Agris : F60 - Physiologie et biochimie végétale
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Hors axes (2014-2018)

Auteurs et affiliations

• Brillouet Jean-Marc, INRA (FRA)
• Romieu Charles
• Lartaud Marc, CIRAD-BIOS-UMR AGAP (FRA)
• Jublanc Elodie, INRA (FRA)
• Torregrosa Laurent
• Cazevieille Chantal, Centre Régional d'Imagerie Cellulaire (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/574717/)

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