Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

Song Zhongshu, Bakeer Walid, Marshall James W., Yakasai Ahmed A., Mohd Khalid Rozida, Collemare Jérôme, Skellam Elizabeth, Tharreau Didier, Lebrun Marc-Henri, Lazarus Colin M., Bailey Andrew M., Simpson Thomas J., Cox Russel J.. 2015. Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. Chemical Science, 6 (8) : 4837-4845.

Quartile : Outlier, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Résumé : The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.

Mots-clés Agrovoc : Oryza sativa, Magnaporthe, expression des gènes, avirulence, interactions biologiques, relation hôte pathogène, résistance aux maladies

Mots-clés complémentaires : Magnaporthe oryzae

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Song Zhongshu, University of Bristol (GBR)
• Bakeer Walid, University of Bristol (GBR)
• Marshall James W., University of Bristol (GBR)
• Yakasai Ahmed A., University of Bristol (GBR)
• Mohd Khalid Rozida, University of Bristol (GBR)
• Collemare Jérôme, INRA (FRA)
• Skellam Elizabeth, University of Hannover (DEU)
• Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120
• Lebrun Marc-Henri, INRA (FRA)
• Lazarus Colin M., University of Bristol (GBR)
• Bailey Andrew M., University of Bristol (GBR)
• Simpson Thomas J., University of Bristol (GBR)
• Cox Russel J., University of Bristol (GBR)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/577195/)

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