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Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection

Legavre Thierry, Ducamp Michel, Sabau Xavier, Argout Xavier, Fouet Olivier, Dedieu Frédéric, Surujdeo-Maharaj Surendra, Garcia Dominique, Paulin Didier, Lanaud Claire. 2015. Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection. Physiological and Molecular Plant Pathology, 92 : pp. 1-13.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : PLANT SCIENCES

Abstract : Cocoa black pod caused by Phytophthora is one of the most serious diseases affecting cocoa production. This project set out was to improve our knowledge of the molecular mechanisms involved in Theobroma cacao resistance. We performed a transcriptional screening using macroarrays containing 89 ESTs from subtractive libraries enriched with genes differentially expressed in response to T. cacao/Phytophthora interactions. A gene expression analysis revealed the induction of genes involved in stress signal transduction, general and specific defence genes and genes involved in hormone signalling pathways. RT-qPCR showed that the level expression of two genes in the resistant plant was higher during the first eight hours after pathogen inoculation. Three genes from a family encoding a protease inhibitor displayed different levels of expression depending on resistant and susceptible T. cacao clones. A comparison of promoter sequences revealed a motif present only in genes that were up-expressed in the resistant plant. (Résumé d'auteur)

Mots-clés Agrovoc : Theobroma cacao, Phytophthora megasperma, Résistance génétique, Expression des gènes, Relation hôte pathogène, Inhibiteur d'enzyme, Protéase, PCR, Hybridation, Physiologie végétale, Gène

Mots-clés géographiques Agrovoc : Papouasie-Nouvelle-Guinée, Trinité-et-Tobago, France

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases
F60 - Plant physiology and biochemistry

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Legavre Thierry, CIRAD-BIOS-UMR AGAP (FRA)
  • Ducamp Michel, CIRAD-BIOS-UMR BGPI (FRA)
  • Sabau Xavier, CIRAD-BIOS-UMR AGAP (FRA)
  • Argout Xavier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-0100-5511
  • Fouet Olivier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8547-1474
  • Dedieu Frédéric, CIRAD-BIOS-UPR Bioagresseurs : analyse et maîtrise du risque (FRA)
  • Surujdeo-Maharaj Surendra, UWI (TTO)
  • Garcia Dominique, CIRAD-BIOS-UMR AGAP (FRA)
  • Paulin Didier
  • Lanaud Claire, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6411-7310

Source : Cirad-Agritrop (https://agritrop.cirad.fr/577414/)

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