Agritrop
Home

Development of a new pCAMBIA binary vector using Gateway® technology

Leclercq Julie, Szabolcs Toth, Martin Florence, Montoro Pascal. 2015. Development of a new pCAMBIA binary vector using Gateway® technology. Plasmid, 81 : pp. 50-54.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
1-s2.0-S0147619X15300068-main.pdf

Télécharger (415kB) | Request a copy

Quartile : Q3, Sujet : MICROBIOLOGY / Quartile : Q4, Sujet : GENETICS & HEREDITY

Abstract : pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper sets out to convert the pCambia2300 binary vector into a destination vector with the Gateway® cassette driven by the CaMV35S promoter. The destination vector, pCamway35S, was then evaluated using the uidA reporter gene. Transient and stable transformation experiments were successfully assayed, either by particle bombardment or by Agrobacterium tumefaciens in Allium cepa and Hevea embryogenic calli. After counting the transformation units, the statistical analysis performed on the data showed that the pCamway 35S::uidA vector was as efficient as pCambia2301, a pCAMBIA2300 containing the uidA reporter gene under the CaMV 35S promoter. (Résumé d'auteur)

Mots-clés Agrovoc : Transformation génétique, Génie génétique, Fluorescence, Protéine, Hevea brasiliensis, Agrobacterium tumefaciens, Allium cepa, Plante transgénique

Mots-clés géographiques Agrovoc : France

Classification Agris : F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
  • Szabolcs Toth
  • Martin Florence, CIRAD-BIOS-UMR AGAP (FRA)
  • Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578294/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2020-10-13 ]