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Worldwide emergence of colistin resistance in Klebsiella pneumoniae from healthy humans and patients in Lao PDR, Thailand, Israel, Nigeria and France owing to inactivation of the PhoP/PhoQ regulator mgrB: an epidemiological and molecular study

Olaitan Abiola Olumuyiwa, Diene Seydina M., Kempf Marie, Berrazeg Meryem, Bakour Sofiane, Gupta Sushim Kumar, Thongmalayvong Boupha, Akkhavong Kongsap, Somphavong Silaphet, Paboriboune Phimpha, Chaisiri Kittipong, Komalamisra Chalit, Adelowo Olawale Olufemi, Fagade Obasola Ezekiel, Banjo Omowunmi Abosede, Oke Adeyeye James, Adler Amos, Assous Marc Victor, Morand Serge, Raoult Didier, Rolain Jean-Marc. 2014. Worldwide emergence of colistin resistance in Klebsiella pneumoniae from healthy humans and patients in Lao PDR, Thailand, Israel, Nigeria and France owing to inactivation of the PhoP/PhoQ regulator mgrB: an epidemiological and molecular study. International Journal of Antimicrobial Agents, 44 (6) : pp. 500-507.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Url - jeu de données : http://www.ebi.ac.uk/ena/data/view/PRJEB1319 / Url - jeu de données : http://www.ebi.ac.uk/ena/data/view/PRJEB4964 / Url - jeu de données : http://www.ncbi.nlm.nih.gov/nuccore/KF852758 / Url - jeu de données : http://www.ncbi.nlm.nih.gov/nuccore/KF852771

Quartile : Q1, Sujet : PHARMACOLOGY & PHARMACY / Quartile : Q1, Sujet : MICROBIOLOGY / Quartile : Q1, Sujet : INFECTIOUS DISEASES

Additional Information : Jeux de données enregistrés dans la base GenBank du numéro d'accès KF852758 au KF852771.

Abstract : The emergence of colistin-resistant Klebsiella pneumoniae (CRKP) is a major public health concern worldwide. In this study, the prevalence and molecular basis of colistin resistance in CRKP isolated from healthy individuals and patients in Lao PDR, Thailand, Nigeria and France were investigated. Stool samples were screened by culture for the presence of colistin-resistant Klebsiella spp. Whole-genome sequence analysis was used to decipher the molecular mechanism of colistin resistance in a blaNDM-1-positive in vitro-selected CRKP mutant. PCR amplification and sequencing of the mgrB genetic environment was performed for all CRKP isolates as well as control colistin-susceptible K. pneumoniae (CSKP) isolates recovered from the same stools. A total of 869 stool samples were screened for colistin-resistant Klebsiella spp., yielding 32 CRKP and 2 colistin-resistant Klebsiella oxytoca. Comparative whole-genome sequence analysis revealed that an in vitro-selected CRKP mutant had an insertion sequence in its mgrB gene, as well as missense mutations in other selected clones. Of the 34 colistin-resistant Klebsiella spp. isolates, 14 (41.2%; 13 CRKP and 1 K. oxytoca) from the four countries also had various defects in their mgrB genes, but no such defects were found in the CSKP controls (P < 10−4). Few mutations were observed in pmrAB compared with mgrB among the CRKP isolates. The worldwide emergence of CRKP is a major public health concern. Detection and surveillance of such strains are warranted to prevent an uncontrollable pandemic. Inactivation of the PhoP/PhoQ regulator gene mgrB is associated with ≥40% of colistin resistance among the CRKP isolates observed in this study. (Résumé d'auteur)

Mots-clés géographiques Agrovoc : République démocratique populaire lao, Thaïlande, Nigéria, France

Mots-clés libres : Antibiotic resistance, Colistin resistance, Genome analysis, Transposon, Two-component systems, MgrB gene

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Olaitan Abiola Olumuyiwa, CNRS (FRA)
  • Diene Seydina M., CNRS (FRA)
  • Kempf Marie, CHU Angers (FRA)
  • Berrazeg Meryem, CNRS (FRA)
  • Bakour Sofiane, CNRS (FRA)
  • Gupta Sushim Kumar, CNRS (FRA)
  • Thongmalayvong Boupha, NIOPH (LAO)
  • Akkhavong Kongsap, NIOPH (LAO)
  • Somphavong Silaphet, CICML (LAO)
  • Paboriboune Phimpha, CICML (LAO)
  • Chaisiri Kittipong, Mahidol University (THA)
  • Komalamisra Chalit, Mahidol University (THA)
  • Adelowo Olawale Olufemi, University of Ibadan (NGA)
  • Fagade Obasola Ezekiel, University of Ibadan (NGA)
  • Banjo Omowunmi Abosede, University of Ibadan (NGA)
  • Oke Adeyeye James, Bowen university (NGA)
  • Adler Amos, Ministère de la santé (Israël) (ISR)
  • Assous Marc Victor, Shaare Zedek Medical Center (ISR)
  • Morand Serge, CIRAD-ES-UPR AGIRs (LAO) ORCID: 0000-0003-3986-7659
  • Raoult Didier, CNRS (FRA)
  • Rolain Jean-Marc, Faculté de Médecine et de Pharmacie (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578448/)

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