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Hydroxycinnamic acid degradation, a broadly conserved trait, protects Ralstonia solanacearum from chemical plant defenses and contributes to root colonization and virulence

Lowe Tiffany, Ailloud Florent, Allen Caitilyn. 2015. Hydroxycinnamic acid degradation, a broadly conserved trait, protects Ralstonia solanacearum from chemical plant defenses and contributes to root colonization and virulence. Molecular Plant-Microbe Interactions, 28 (3) : pp. 286-297.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Plants produce hydroxycinnami c acid (HCA) defense com- pounds to combat pathogens, such as the bacterium Ral- stonia solanacearum . We showed that an HCA degradation pathway is genetically and functionally conserved across diverse R. solanacearum strains. Further, a feruloyl-CoA synthetase ( Δ fcs ) mutant that canno t degrade HCA was less virulent on tomato plants. To understand the role of HCA degradation in bacterial wilt disease, we tested the following hypotheses: HCA degradation helps the patho- gen i) grow, as a carbon source; ii) spread, by reducing HCA-derived physical barriers; and iii) survive plant anti- microbial compounds. Although HCA degradation enabled R. solanacearum growth on HCA in vitro, HCA degrada- tion was dispensable for growth in xylem sap and root exu- date, suggesting that HCA are not significant carbon sources in planta. Acetyl-bromide quantification of lignin demonstrated that R. solanacearum infections did not affect the gross quantity or distribution of stem lignin. However, the Δ fcs mutant was significantly more suscepti- ble to inhibition by two HCA, namely, caffeate and p -couma- rate. Finally, plant colonization assays suggested that HCA degradation facilitates early stages of infection and root colonization. Together, these results indicated that ability to degrade HCA contributes to bacterial wilt virulence by facilitating root entry and by protecting the pathogen from HCA toxicity.

Mots-clés Agrovoc : Ralstonia solanacearum, mécanisme de défense, Acide hydroxycinnamique, Biodégradation, Relation hôte pathogène, Colonisation des racines, Virulence, Solanum lycopersicum, Escherichia coli

Mots-clés géographiques Agrovoc : Réunion

Classification Agris : H20 - Plant diseases
F60 - Plant physiology and biochemistry

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Lowe Tiffany, University of Wisconsin (USA)
  • Ailloud Florent, CIRAD-BIOS-UMR PVBMT (REU)
  • Allen Caitilyn, University of Wisconsin (USA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578594/)

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