Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis

Dessailly Florence, Besrest Pierre, Martin Florence, Lardet Ludovic, Granet Françoise, Rio Maryannick, Montoro Pascal. 2015. Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis. . Phuket : Prince of Songkla University, 4 p. Asia Pacific Rubber Conference. 2, Phuket, Thaïlande, 15 Octobre 2015/16 Octobre 2015.

Résumé : Hevea brasiliensisis cloned by grafting on non-selected rootstock material. In-vitro techniques such as microcutting and somatic embryogenesis were used for propagation of self-rooted plant material. However, these non-commercial techniques are still restricted to a few numbers of clones. To date, long-term somatic embryogenesis is the most promising technique for large-scale propagation. At CIRAD, successful plant regeneration and genetic modification have been obtained for clone PB 260. This paper aims at developing and cryopreserving embryogenic callus lines for clone RRIM 600. Culture conditions were modified for suitable callus growth and embryogenic induction of the low multiplication rate of RRIM600 callus compared with the clone PB 260 one. Several friable callus lines with high embryogenic potential were selected and cryopreserved in large quantity for further field test and functional genomics studies. Application of this technology to other commercial clones can be now considered based on this experience on clones PB 260 and RRIM 600.

Mots-clés libres : Hevea brasiliensis, Cryo preservation, Somatic embryogenesis, Genetic modification, Plant regeneration, Rubber

Classification Agris : F02 - Multiplication végétative des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Dessailly Florence, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-7873-3974
• Besrest Pierre
• Martin Florence, CIRAD-BIOS-UMR AGAP (FRA)
• Lardet Ludovic, CIRAD-BIOS-UMR AGAP (FRA)
• Granet Françoise, Michelin (FRA)
• Rio Maryannick, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-4573-1371
• Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/579154/)

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