Waithaisong Kittima, Robin Agnès, Martin Agnès, Clairotte Michael, Villeneuve Manon, Plassard Claude. 2015. Quantification of organic P and low-molecular-weight organic acids in soil extracts by high performance ion chromatograph. Geoderma, 257-258 : 94-101.
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Quartile : Q1, Sujet : SOIL SCIENCE
Résumé : A better understanding of the composition of organic phosphorus (organic P) and low-molecular-weight organic acids (LMWOAs) in soil will make it possible to predict phosphorus cycling, improve biomass production and help phosphorus management in forest plantations. The method for quantifying organic P and LMWOAs in soil extracts was developed using ion chromatography (IC). Sodium hydroxide (0.5 N) was validated as an extractant for both organic P and LMWOAs. A mixture of 200 mM NaOH and ultrapure water was used to create a linear gradient elution. The eluent program separated five different organic P forms (glucose-6-phosphate, AMP, fructose-1,6-bisphosphate, ATP and phytate) as well as pyrophosphate from inorganic P (Pi), and four LMWOAs (malate, malonate, oxalate and citrate) in a 25 minute run through an AS11 column. The variability of the quantification was expressed as the relative standard deviation which was less than 20% for most of the analytes. The recovery rate of organic P assayed by IC to colorimetry was 110 ± 20%. This chromatographic method was shown to be suitable for the quantification of organic P and LMWOAs in soil samples and could be an easier alternative to 31P Nuclear Magnetic Resonance (NMR) measurement.
Mots-clés Agrovoc : fertilité du sol, phosphore, acide organique, aménagement forestier, plantation forestière, biomasse, cycle du phosphore, production de biomasse
Classification Agris : P33 - Chimie et physique du sol
Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive
Auteurs et affiliations
- Waithaisong Kittima, INRA (FRA)
- Robin Agnès, CIRAD-PERSYST-UMR Eco&Sols (BRA)
- Martin Agnès, CIRAD-PERSYST-UMR Eco&Sols (FRA)
- Clairotte Michael, INRA (FRA)
- Villeneuve Manon, IRD (FRA)
- Plassard Claude, INRA (FRA)
Source : Cirad-Agritrop (https://agritrop.cirad.fr/579392/)
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