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Escherichia coli population structure and antibiotic resistance at a buffalo/cattle interface in Southern Africa

Mercat Mathilde, Clermont Olivier, Massot Méril, Ruppe Etienne, De Garine-Wichatitsky Michel, Miguel Eve, Valls Fox Hugo, Cornélis Daniel, Andremont Antoine, Denamur Erick, Caron Alexandre. 2016. Escherichia coli population structure and antibiotic resistance at a buffalo/cattle interface in Southern Africa. Applied and Environmental Microbiology, 82 (5) : pp. 1459-1467.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q1, Sujet : MICROBIOLOGY

Abstract : At a human/livestock/wildlife interface, Escherichia coli populations were used to assess the risk of bacterial and antibiotic resistance dissemination between hosts. We used phenotypic and genotypic characterization techniques to describe the structure and the level of antibiotic resistance of E. coli commensal populations and the resistant Enterobacteriaceae carriage of sympatric African buffalo (Syncerus caffer caffer) and cattle populations characterized by their contact patterns in the southern part of Hwange ecosystem in Zimbabwe. Our results (i) confirmed our assumption that buffalo and cattle share similar phylogroup profiles, dominated by B1 (44.5%) and E (29.0%) phylogroups, with some variability in A phylogroup presence (from 1.9 to 12%); (ii) identified a significant gradient of antibiotic resistance from isolated buffalo to buffalo in contact with cattle and cattle populations expressed as the Murray score among Enterobacteriaceae (0.146, 0.258, and 0.340, respectively) and as the presence of tetracycline-, trimethoprim-, and amoxicillin-resistant subdominant E. coli strains (0, 5.7, and 38%, respectively); (iii) evidenced the dissemination of tetracycline, trimethoprim, and amoxicillin resistance genes (tet, dfrA, and blaTEM-1) in 26 isolated subdominant E. coli strains between nearby buffalo and cattle populations, that led us (iv) to hypothesize the role of the human/animal interface in the dissemination of genetic material from human to cattle and toward wildlife. The study of antibiotic resistance dissemination in multihost systems and at anthropized/natural interface is necessary to better understand and mitigate its multiple threats. These results also contribute to attempts aiming at using E. coli as a tool for the identification of pathogen transmission pathway in multihost systems. (Résumé d'auteur)

Mots-clés Agrovoc : Escherichia coli, Résistance aux antibiotiques, Génétique des populations, Buffle africain, Bétail, Bovin, Transmission des maladies, Organisme indicateur, Maladie de l'homme, Écologie microbienne, Animal sauvage, Animal domestique, Interactions biologiques

Mots-clés géographiques Agrovoc : Afrique australe, Zimbabwe

Mots-clés libres : Escherichia coli, Antibiotic resistance, Buffalo-cattle interface, Southern Africa

Classification Agris : L73 - Animal diseases
000 - Autres thèmes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Mercat Mathilde, INSERM (FRA)
  • Clermont Olivier, INSERM (FRA)
  • Massot Méril, INSERM (FRA)
  • Ruppe Etienne, INSERM (FRA)
  • De Garine-Wichatitsky Michel, CIRAD-ES-UPR AGIRs (ZWE) ORCID: 0000-0002-5438-1473
  • Miguel Eve, Université de Montpellier (FRA)
  • Valls Fox Hugo, CNRS (ZWE)
  • Cornélis Daniel, CIRAD-ES-UPR AGIRs (ZWE)
  • Andremont Antoine, Université de Paris VII (FRA)
  • Denamur Erick, INSERM (FRA)
  • Caron Alexandre, CIRAD-ES-UPR AGIRs (ZWE) ORCID: 0000-0002-5213-3273

Source : Cirad-Agritrop (https://agritrop.cirad.fr/579794/)

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