Embryogenic potential of hybrids originated from Coffea arabica (Caturra X Timor Hybrid) X wild Ethiopian accessions crosses (Pb212)

Villegas A.M., Gongora C., Posada S.H., Bertrand Benoît, Gast F.. 2015. Embryogenic potential of hybrids originated from Coffea arabica (Caturra X Timor Hybrid) X wild Ethiopian accessions crosses (Pb212). In : Proceedings of the 25th International Conference on Coffee Science. ASIC. Paris : ASIC, 42-47. ISBN 978-2-900212-24-0 International Conference on Coffee Science. 25, Armenia, Colombie, 8 Septembre 2014/13 Septembre 2014.

Résumé : Embryogenic potential is a key trait for the multiplication of promising hybrid genotypes of coffee for experimental and commercial purposes. Therefore, the embryogenic potential was determined in 29 two year-old hybrids resulting from crosses between 10 wild C. arabica accessions from Ethiopia with 3 maternal lines (CU1842, CX2385 and CX2848) obtained from crosses between C. arabica var. Caturra and the Timor Hybrid. First, 100 to 200 leaf explants from each hybrid were planted on NPC media (NPC salts, 2.4D and 2IP); one month later, the explants were transferred to Embryogenic Tissue (ET) inducer medium (MS salts, 2,4D and BAP), with ET regeneration starting after six months, followed by subculture of regenerated tissues in either Liquid Proliferation Medium (MS salts, 2,4 D and Kinetin) or Embryo Liquid differentiation Medium (MS salts, ANA and Kinetin). ET regeneration as well as embryo and plantlet production were observed in 65% of the hybrids planted (19 out of 29). Hybrids derived from the maternal line CX2848 showed the highest regeneration potential, with 8 out of 8 derived hybrids regenerated. Next was line CU1842, with 6 out of 10 hybrids showing regeneration, and last was line CX2385, where only 5 out of 11 hybrids have regenerated. These results are probably associated to the maternal line ancestry and to the introgression level from the Timor Hybrid, where line CX2385 had the largest introgression levels as determined by microsatellite markers, followed by line CU1842, both lines sharing a common ancestor at the F1 generation. The smallest introgression levels were present in line CX2848, that comes from an unrelated cross.

Classification Agris : F30 - Génétique et amélioration des plantes
F02 - Multiplication végétative des plantes

Auteurs et affiliations

• Villegas A.M., CENICAFE (COL)
• Gongora C., CENICAFE (COL)
• Posada S.H., CENICAFE (COL)
• Bertrand Benoît, CIRAD-BIOS-UMR IPME (FRA) ORCID: 0000-0003-1969-3479
• Gast F., CENICAFE (COL)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/579990/)

Métriques

[ Page générée et mise en cache le 2024-03-27 ]