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Epidemiological surveys of camel trypanosomosis in Al-jouf, Saudi Arabia based on PCR and ELISA

El Wathig Mohamed, Faye Bernard, Thevenon Sophie, Ravel Sophie, Bossard Géraldine. 2016. Epidemiological surveys of camel trypanosomosis in Al-jouf, Saudi Arabia based on PCR and ELISA. Emirates Journal of Food and Agriculture, 28 (3) : pp. 212-216.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q3, Sujet : AGRONOMY / Quartile : Q4, Sujet : FOOD SCIENCE & TECHNOLOGY

Abstract : Trypanosomosis due to Trypanosoma evansi (surra) is a major enzootic disease of the dromedary camel. The present study was conducted to determine the prevalence of camel trypanosomosis in the northern part of Saudi Arabia with different methods of diagnosis (ELISA, PCR) and to compare the results to whose obtained previously with card Agglutination test (CATT/T.evansi). A total of 195 blood samples and 118 serum samples were used for molecular and serological investigation respectively. After analyses, 25% (49/195) and 3% (4/118) samples were positive using PCR and ELISA respectively. The variability of trypanosomosis was highly significant to the factor moving, location, breed and clinical signs with PCR. The discrepancy between PCR, CATT test and ELISA is likely due to antibodies degradation on spotted papers maintained several at ambient temperature. This is the first molecular diagnosis report, which gives a picture of camel trypanosomiasis in Al-jouf, Saudi Arabia. (Résumé d'auteur)

Mots-clés Agrovoc : Chameau, Trypanosoma evansi, Immunodiagnostic, PCR, Test ELISA, Technique immunologique, Agglutination immunologique, Morbidité, Facteur de risque, Épidémiologie, Sang, Sérum sanguin, Enquête pathologique

Mots-clés géographiques Agrovoc : Arabie Saoudite

Mots-clés libres : Trypanosoma evansi, Camels, PCR, ELISA, Risk factors, Al-jouf

Classification Agris : L73 - Animal diseases
U40 - Surveying methods
L72 - Pests of animals

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • El Wathig Mohamed, CIRAD-ES-UMR SELMET (FRA)
  • Faye Bernard, CIRAD-ES-UMR SELMET (FRA)
  • Thevenon Sophie, CIRAD-BIOS-UMR INTERTRYP (FRA)
  • Ravel Sophie, IRD (FRA)
  • Bossard Géraldine, CIRAD-BIOS-UMR INTERTRYP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580075/)

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