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Evaluation of an indirect-ELISA test for Trypanosoma evansi infection (Surra) in buffaloes and its application to a serological survey in Thailand

Kocher Arthur, Desquesnes Marc, Kamyingkird Ketsarin, Yangtara Sarawut, Leboucher Emilye, Rodtian Pranee, Dargantes Alan, Jittapalapong Sathaporn. 2015. Evaluation of an indirect-ELISA test for Trypanosoma evansi infection (Surra) in buffaloes and its application to a serological survey in Thailand. BioMed Research International, 2015 (ID 361037), 8 p.

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Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q3, Sujet : MEDICINE, RESEARCH & EXPERIMENTAL

Abstract : Surra, caused by Trypanosoma evansi, is a neglected disease due to frequent subclinical evolution, especially in bovines in Asia. However, acute and chronic signs are regularly observed, with significant sanitary and economic impacts. In this study, we evaluated and applied an indirect-ELISA test for the detection of anti-T. evansi immunoglobulin G in buffaloes using antibovine conjugate. Based on buffalo reference sera from the Philippines, a two-graph receiver operating characteristics analysis (TG-ROC) was conducted to define an optimal cut-off value; sensitivity and specificity were estimated at 92.5% and 94.2%, respectively. A cross-sectional serological survey was carried out in the major buffalo breeding areas of Thailand; 892 buffaloes from 8 provinces were sampled in North, Northeastern, and Southern Thailand. Seropositive buffaloes were found in all 8 provinces, on 20.3% of farms for an overall prevalence of 12.2% (95% CI 10.2–14.5%). Nearly one-third of the sampled population was exposed to infection. Broader sampling would be necessary but is not possible in the southern half-wild breeding systems. According to our results, buffaloes may constitute a large and robust reservoir for T. evansi, which is a permanent threat to other livestock such as cattle and horses as well as wild animals such as elephants in Southest Asia. (Résumé d'auteur)

Mots-clés Agrovoc : Trypanosoma evansi, Surra, Buffle africain, Test ELISA, Immunodiagnostic, Sérologie, Enquête pathologique

Mots-clés géographiques Agrovoc : Thaïlande, Philippines

Classification Agris : L72 - Pests of animals

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Kocher Arthur, Kasetsart University (THA)
  • Desquesnes Marc, CIRAD-BIOS-UMR INTERTRYP (THA) ORCID: 0000-0002-7665-2422
  • Kamyingkird Ketsarin, Kasetsart University (THA)
  • Yangtara Sarawut, Kasetsart University (THA)
  • Leboucher Emilye, Kasetsart University (THA)
  • Rodtian Pranee, Department of Livestock Development (THA)
  • Dargantes Alan, Central Mindanao University (PHL)
  • Jittapalapong Sathaporn, Kasetsart University (THA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580286/)

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