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Cryptosporidium genotypes in children and calves living at the wildlife- livestock interface of the Kruger National Park, South Africa

Abu Samra Nada, Jori Ferran, Caccio Simone M., Frean John, Poonsamy Bhavani, Thompson Peter. 2016. Cryptosporidium genotypes in children and calves living at the wildlife- livestock interface of the Kruger National Park, South Africa. Onderstepoort Journal of Veterinary Research, 83 (1), 7 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q3, Sujet : VETERINARY SCIENCES

Abstract : Cryptosporidium infection is one of the most common causes of parasitic diarrhoea worldwide in cattle and humans. In developing countries, human cryptosporidiosis is most prevalent during early childhood and links between zoonotic infection and animal related activities have been demonstrated. This study investigated the prevalence and species/genotype distribution of Cryptosporidium among children (< 5 years) and calves (< 6 months) living in a rural farming area adjacent to the Kruger National Park in South Africa, where interactions between humans and wild and domestic animals are known to occur. Cryptosporidium oocysts were detected in 8/143 stool samples of children recruited within the hospital system (5.6%; 95% CI 2.4%, 10.7%) and in 2/352 faecal samples of calves (0.6%; 95% CI 0.1%, 2.0%) using the modified Ziehl–Neelsen (MZN) staining technique. Microscopy positive samples from children were further analysed by PCR targeting the 18S rRNA gene and identified as Cryptosporidium hominis (3/4) and Cryptosporidium meleagridis (1/4). Regardless of the microscopy outcome, randomly selected samples (n = 36) from calves 0–4 months of age were amplified and sequenced at the 18S rRNA gene using nested PCR. Two calves tested positive (5.6%; 95% CI 1.7%, 18.7%), and revealed the presence of Cryptosporidium parvum and Cryptosporidium bovis. The detection of only two zoonotic species (C. parvum in one calf and C. meleagridis in one child) suggests that zoonotic cryptosporidiosis is not currently widespread in our study area; however, the potential exists for amplification of transmission in an immunocompromised population. (Résumé d'auteur)

Mots-clés Agrovoc : Cryptosporidium, Génotype, Variation génétique, Morbidité, Analyse du risque, Enfant, Veau, Animal sauvage, Parc national, Interactions biologiques, Maladie de l'homme, Maladie des animaux, zoonose, Cryptosporidium parvum

Mots-clés géographiques Agrovoc : Afrique du Sud

Mots-clés complémentaires : Cryptosporidium meleagridis, Cryptosporidium bovis

Mots-clés libres : Cryptosporidium, Children, Calves, South Africa, Genotyping

Classification Agris : L72 - Pests of animals
L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Abu Samra Nada, University of Pretoria (ZAF)
  • Jori Ferran, CIRAD-ES-UPR AGIRs (BWA) ORCID: 0000-0001-5451-7767
  • Caccio Simone M., Istituto Superiore di Sanita (ITA)
  • Frean John, University of the Witwatersrand (ZAF)
  • Poonsamy Bhavani, National Institute for Communicable Diseases (ZAF)
  • Thompson Peter, University of Pretoria (ZAF)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580512/)

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