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Characterization of microsatellites identified by next-generation sequencing in the neotropical tree Handroanthus billbergii (Bignoniaceae)

Morillo Velastegui Luis Eduardo, Buitron Johanna, Limongi Ricardo, Vignes Hélène, Argout Xavier. 2016. Characterization of microsatellites identified by next-generation sequencing in the neotropical tree Handroanthus billbergii (Bignoniaceae). Applications in Plant Sciences, 4 (5):1500135, 4 p.

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Quartile : Q2, Sujet : PLANT SCIENCES

Abstract : Premise of the study: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador. Methods and Results: Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii. Conclusions: We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species. (Résumé d'auteur)

Mots-clés Agrovoc : Tabebuia, Bignoniaceae, Marqueur génétique, ADN, Biodiversité, forêt tropicale, Écosystème forestier, Variation génétique, Polymorphisme génétique, Séquence d'ADN

Mots-clés géographiques Agrovoc : Équateur

Mots-clés complémentaires : Handroanthus billbergii

Classification Agris : F30 - Plant genetics and breeding
K01 - Forestry - General aspects
F40 - Plant ecology

Champ stratégique Cirad : Axe 6 (2014-2018) - Sociétés, natures et territoires

Auteurs et affiliations

  • Morillo Velastegui Luis Eduardo, CIRAD-BIOS-UMR AGAP (FRA)
  • Buitron Johanna, INIAP (ECU)
  • Limongi Ricardo, INIAP (ECU)
  • Vignes Hélène, CIRAD-BIOS-UMR AGAP (FRA)
  • Argout Xavier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-0100-5511

Source : Cirad-Agritrop (https://agritrop.cirad.fr/581136/)

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