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Biodiesel production from crude jatropha oil catalyzed by immobilized lipase/acyltransferase from Candida parapsilosis in aqueous medium

Rodrigues Joana, Perrier Véronique, Lecomte Jérôme, Dubreucq Eric, Ferreira-Dias Suzana. 2016. Biodiesel production from crude jatropha oil catalyzed by immobilized lipase/acyltransferase from Candida parapsilosis in aqueous medium. Bioresource Technology, 218 : pp. 1224-1229.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : AGRICULTURAL ENGINEERING / Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q1, Sujet : ENERGY & FUELS

Abstract : The lipase/acyltransferase from Candida parapsilosis (CpLIP2) immobilized on two synthetic resins (Accurel MP 1000 and Lewatit VP OC 1600) was used as catalyst for the production of biodiesel (fatty acid methyl esters, FAME) by transesterification of jatropha oil with methanol, in a lipid/aqueous system. The oil was dispersed in a buffer solution (pH 6.5) containing methanol in excess (2 M in the biphasic system; molar ratio methanol/acyl chains 2:1). Transesterification was carried out at 30 °C, under magnetic stirring, using 10% (w/w) of immobilized enzyme in relation to oil. The maximum FAME yields were attained after 8 h reaction time: 80.5% and 93.8%, when CpLIP2 immobilized on Accurel MP 1000 or on Lewatit VP OC 1600 were used, respectively. CpLIP2 on both Accurel MP 1000 and Lewatit VP OC 1600 showed high operational stability along 5 consecutive 8 h batches. (Résumé d'auteur)

Mots-clés libres : Lipase/acyltransferase, Biodiesel, Candida parapsilosis, CpLIP2, Jatropha oil, Operational stability

Classification Agris : P06 - Renewable energy resources
Q60 - Processing of non-food or non-feed agricultural products

Champ stratégique Cirad : Axe 2 (2014-2018) - Valorisation de la biomasse

Auteurs et affiliations

  • Rodrigues Joana, University of Lisbon (PRT)
  • Perrier Véronique, Montpellier SupAgro (FRA)
  • Lecomte Jérôme, CIRAD-PERSYST-UMR IATE (FRA)
  • Dubreucq Eric, Montpellier SupAgro (FRA)
  • Ferreira-Dias Suzana, University of Lisbon (PRT)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/581207/)

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