Gene coexpression network analysis of oil biosynthesis in an interspecific backcross of oil palm

Guérin Chloé, Joët Thierry, Serret Julien, Lashermes Philippe, Vaissayre Virginie, Agbessi Mawussé D.T., Beulé Thierry, Severac Dany, Amblard Philippe, Tregear James, Durand-Gasselin Tristan, Morcillo Fabienne, Dussert Stéphane. 2016. Gene coexpression network analysis of oil biosynthesis in an interspecific backcross of oil palm. Plant Journal, 87 (5) : 423-441.

https://doi.org/10.1111/tpj.13208

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

Prévisualisation

Version Online first - Anglais
Sous licence CC0 1.0 Sans restriction de droits pour le monde entier.
Guerin_et_al-2016-The_Plant_Journal.pdf
Télécharger (2MB) | Prévisualisation

Prévisualisation

Version publiée - Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad.
Guerin_et_al-2016-The_Plant_Journal.pdf
Télécharger (2MB) | Prévisualisation

Quartile : Outlier, Sujet : PLANT SCIENCES

Résumé : Global demand for vegetable oils is increasing at a dramatic rate, while our understanding of the regulation of oil biosynthesis in plants remains limited. To gain insights into the mechanisms that govern oil synthesis and fatty acid (FA) composition in the oil palm fruit, we used a multilevel approach combining gene coexpression analysis, quantification of allele-specific expression and joint multivariate analysis of transcriptomic and lipid data, in an interspecific backcross population between the African oil palm, Elaeis guineensis, and the American oil palm, Elaeis oleifera, which display contrasting oil contents and FA compositions. The gene coexpression network produced revealed tight transcriptional coordination of fatty acid synthesis (FAS) in the plastid with sugar sensing, plastidial glycolysis, transient starch storage and carbon recapture pathways. It also revealed a concerted regulation, along with FAS, of both the transfer of nascent FA to the endoplasmic reticulum, where triacylglycerol assembly occurs, and of the production of glycerol-3-phosphate, which provides the backbone of triacylglycerols. Plastid biogenesis and auxin transport were the two other biological processes most tightly connected to FAS in the network. In addition to WRINKLED1, a transcription factor (TF) known to activate FAS genes, two novel TFs, termed NF-YB-1 and ZFP-1, were found at the core of the FAS module. The saturated FA content of palm oil appeared to vary above all in relation to the level of transcripts of the gene coding for β-ketoacyl-acyl carrier protein synthase II. Our findings should facilitate the development of breeding and engineering strategies in this and other oil crops.

Mots-clés Agrovoc : Elaeis guineensis, Elaeis oleifera, hybridation interspécifique, biosynthèse, huile de palme, acide gras, acide palmitique, glycolyse, métabolisme des lipides, amidon, métabolisme des glucides, Transcription génique, expression des gènes, génome, mésocarpe, génétique des populations