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Rubber particle proteins REF1 and SRPP1 interact differently with native lipids extracted from Hevea brasiliensis latex

Wadeesirisak Kanthida, Castano Sabine, Berthelot Karine, Vaysse Laurent, Bonfils Frédéric, Peruch Frédéric, Rattanaporn Kittipong, Liengprayoon Siriluck, Lecomte Sophie, Bottier Céline. 2017. Rubber particle proteins REF1 and SRPP1 interact differently with native lipids extracted from Hevea brasiliensis latex. Biochimica et Biophysica Acta (BBA). Biomembranes, 1859 (2) : pp. 201-210.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Wadeesirisak_2016.pdf

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Quartile : Q2, Sujet : BIOPHYSICS / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Rubber particle membranes from the Hevea latex contain predominantly two proteins, REF1 and SRPP1 involved in poly(cis-1,4-isoprene) synthesis or rubber quality. The repartition of both proteins on the small or large rubber particles seems to differ, but their role in the irreversible coagulation of the rubber particle is still unknown. In this study we highlighted the different modes of interactions of both recombinant proteins with different classes of lipids extracted from Hevea brasiliensis latex, and defined as phospholipids (PL), glycolipids (GL) and neutral lipids (NL). We combined two biophysical methods, polarization modulated-infrared reflection adsorption spectroscopy (PM-IRRAS) and ellipsometry to elucidate their interactions with monolayers of each class of lipids. REF1 and SRPP1 interactions with native lipids are clearly different; SRPP1 interacts mostly in surface with PL, GL or NL, without modification of its structure. In contrast REF1 inserts deeply in the lipid monolayers with all lipid classes. With NL, REF1 is even able to switch from α-helice conformation to β-sheet structure, as in its aggregated form (amyloid form). Interaction between REF1 and NL may therefore have a specific role in the irreversible coagulation of rubber particles. (Résumé d'auteur)

Mots-clés Agrovoc : Hevea brasiliensis, Latex, Protéine végétale, Fibre végétale, Membrane, Composition chimique, Lipide, Spectroscopie infrarouge, Propriété physicochimique, Coagulation, Polarisation

Mots-clés complémentaires : Nanoparticule

Mots-clés libres : Hevea brasiliensis rubber particle proteins, Rubber elongation factor, Small rubber particle protein, Latex native lipids, Langmuir monolayer, Polarization modulated-infrared reflection absorption spectroscopy

Classification Agris : F60 - Plant physiology and biochemistry
Q60 - Processing of non-food or non-feed agricultural products
U30 - Research methods

Champ stratégique Cirad : Axe 2 (2014-2018) - Valorisation de la biomasse

Auteurs et affiliations

  • Wadeesirisak Kanthida, CIRAD-PERSYST-UMR IATE (FRA)
  • Castano Sabine, CNRS (FRA)
  • Berthelot Karine, CNRS (FRA)
  • Vaysse Laurent, CIRAD-PERSYST-UMR IATE (THA)
  • Bonfils Frédéric, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0001-7016-4770
  • Peruch Frédéric, CNRS (FRA)
  • Rattanaporn Kittipong, KURDI (THA)
  • Liengprayoon Siriluck, CIRAD-PERSYST-UMR IATE (FRA)
  • Lecomte Sophie, CNRS (FRA)
  • Bottier Céline, CIRAD-PERSYST-UMR IATE (THA) ORCID: 0000-0002-5318-4275

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582296/)

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