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A single-step method for RNA isolation from tropical crops in the field

Breitler Jean-Christophe, Campa Claudine, Georget Frederic, Bertrand Benoît, Etienne Hervé. 2016. A single-step method for RNA isolation from tropical crops in the field. Scientific Reports, 6 (38368), 6 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Abstract : The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction method works not only at room temperature but also at elevated temperatures and provides the simplest and most effective single-step method to extract pure and undegraded RNA directly from tropical plants in the field. RNA extraction directly in a complex field environment opens up the way for studying gene-environment interactions at transcriptome level to decipher the complex regulatory network involved in multiple-stress responses. (Résumé d'auteur)

Mots-clés Agrovoc : ARN, Extraction, méthode, Tissu végétal, Coffea arabica, Zea mays, Oryza sativa, Manihot esculenta, Zone tropicale

Mots-clés géographiques Agrovoc : Mexique, Nicaragua, France

Classification Agris : F30 - Plant genetics and breeding
U30 - Research methods
F60 - Plant physiology and biochemistry

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Breitler Jean-Christophe, CIRAD-BIOS-UMR IPME (FRA)
  • Campa Claudine, IRD (FRA)
  • Georget Frederic, CIRAD-BIOS-UMR IPME (CRI)
  • Bertrand Benoît, CIRAD-BIOS-UMR IPME (FRA)
  • Etienne Hervé, CIRAD-BIOS-UMR IPME (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582414/)

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