Agritrop
Home

Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development

Ivamoto Suzana Tiemi, Sakuray Leonardo M., Ferreira Lucia Pires, Kitzberger Cíntia S.G., Scholz Maria Brigida dos Santos, Pot David, Leroy Thierry, Vieira Luiz Gonzaga Esteves, Domingues Douglas S., Pereira Luiz Filipe P.. 2017. Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Plant Physiology and Biochemistry, 111 : pp. 340-347.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
[img] Version Online first - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
pagination_PLAPHY_4744.pdf

Télécharger (1MB) | Request a copy
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
1-s2.0-S0981942816304624-main.pdf

Télécharger (1MB) | Request a copy

Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30–240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. (Résumé d'auteur)

Mots-clés Agrovoc : Coffea arabica, Composition chimique, Tissu végétal, Diterpénoïde, Expression des gènes, Biosynthèse, Développement biologique, PCR, HPLC, Racine, Feuille, Fleur

Mots-clés complémentaires : Cafestol, Kahweol

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry
Q04 - Food composition
U30 - Research methods

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Ivamoto Suzana Tiemi, IAPAR (BRA)
  • Sakuray Leonardo M., IAPAR (BRA)
  • Ferreira Lucia Pires, IAPAR (BRA)
  • Kitzberger Cíntia S.G., IAPAR (BRA)
  • Scholz Maria Brigida dos Santos, IAPAR (BRA)
  • Pot David, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6144-8448
  • Leroy Thierry, CIRAD-BIOS-UMR AGAP (FRA)
  • Vieira Luiz Gonzaga Esteves, IAPAR (BRA)
  • Domingues Douglas S., Universidade de São Paulo (BRA)
  • Pereira Luiz Filipe P., IAPAR (BRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582716/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-03-01 ]