A gene-based map of the Nod factor-independent Aeschynomene evenia sheds new light on the evolution of nodulation and legume genomes

Chaintreuil Clémence, Rivallan Ronan, Bertioli David J., Klopp Christophe, Gouzy Jérôme, Courtois Brigitte, Leleux Philippe, Martin Guillaume, Rami Jean-François, Gully Djamel, Parrinello Hugues, Severac Dany, Patrel Delphine, Fardoux Joël, Ribière William, Boursot Marc, Cartieaux Fabienne, Czernic Pierre, Ratet Pascal, Mournet Pierre, Giraud Eric, Arrighi Jean-François. 2016. A gene-based map of the Nod factor-independent Aeschynomene evenia sheds new light on the evolution of nodulation and legume genomes. DNA Research, 23 (4) : pp. 365-376.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Quartile : Q1, Sujet : GENETICS & HEREDITY

Additional Information : Jeux de données enregistrés dans la base SRA du numéro d'accès SRR3276128, SRR3285082, CIAT8232 et CIAT22838.

Abstract : Aeschynomene evenia has emerged as a new model legume for the deciphering of the molecular mechanisms of an alternative symbiotic process that is independent of the Nod factors. Whereas most of the research on nitrogen-fixing symbiosis, legume genetics and genomics has so far focused on Galegoid and Phaseolid legumes, A. evenia falls in the more basal and understudied Dalbergioid clade along with peanut ( Arachis hypogaea ). To provide insights into the symbiotic genes content and the structure of the A. evenia genome, we established a gene-based genetic map for this species. Firstly, an RNAseq analysis was performed on the two parental lines selected to generate a F 2 mapping population. The transcriptomic data were used to develop molecular markers and they allowed the identification of most symbiotic genes. The resulting map comprised 364 markers arranged in 10 linkage groups (2 n = 20). A comparative analysis with the sequenced genomes of Arachis duranensis and A. ipaensis , the diploid ancestors of peanut, indicated blocks of conserved macrosynteny. Altogether, these results provided important clues regarding the evolution of symbiotic genes in a Nod factor-independent context. They provide a basis for a genome sequencing project and pave the way for forward genetic analysis of symbiosis in A. evenia. (Résumé d'auteur)

Classification Agris : F01 - Crops
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Chaintreuil Clémence, IRD (FRA)
  • Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
  • Bertioli David J., UCB (BRA)
  • Klopp Christophe, INRA (FRA)
  • Gouzy Jérôme, INRA (FRA)
  • Courtois Brigitte, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-2118-7102
  • Leleux Philippe, IRD (FRA)
  • Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500
  • Rami Jean-François, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5679-3877
  • Gully Djamel, IRD (FRA)
  • Parrinello Hugues, Institut de génomique fonctionnelle (FRA)
  • Severac Dany, Institut de génomique fonctionnelle (FRA)
  • Patrel Delphine, IRD (FRA)
  • Fardoux Joël, IRD (FRA)
  • Ribière William, IRD (FRA)
  • Boursot Marc, IRD (FRA)
  • Cartieaux Fabienne, IRD (FRA)
  • Czernic Pierre, IRD (FRA)
  • Ratet Pascal, CNRS (FRA)
  • Mournet Pierre, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8011-8647
  • Giraud Eric, IRD (FRA)
  • Arrighi Jean-François, IRD (FRA)

Source : Cirad-Agritrop (

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