Nguyen Ngoc Thanh Xuan, Sarter Samira, Nguyen N. Hai, Daniel Philippe. 2017. Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 183 : 395-401.
Version publiée
- Anglais
Accès réservé aux personnels Cirad Utilisation soumise à autorisation de l'auteur ou du Cirad. 1-s2.0-S1386142517303566-main.pdf Télécharger (1MB) | Demander une copie |
||
|
Version post-print
- Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad. Xuan 2017-Spectrochimica.pdf Télécharger (1MB) | Prévisualisation |
Quartile : Q1, Sujet : SPECTROSCOPY
Résumé : This study aimed to test Raman (400–1800 cm− 1) and Infra-red (1900–500 cm− 1) spectroscopies followed by statistical analysis (principal component analysis) to detect molecular changes induced by antibiotics (ampicillin, cefotaxime – cell wall synthesis inhibitors, tetracycline – protein synthesis inhibitor, ciprofloxacin – DNA synthesis inhibitor) against Escherichia coli TOP10. In case of ampicillin and cefotaxime, a decrease in protein bands in both Raman (1240, 1660 cm− 1), and IR spectra (1230, 1530, 1630 cm− 1), and an increase in carbohydrate bands (1150, 1020 cm− 1) in IR spectra were observed. Tetracycline addition caused an increase in nucleic acid bands (775, 1478, 1578 cm− 1), a sharp decrease in phenylalanine (995 cm− 1) in Raman spectra and the amide I and amide II bands (1630, 1530 cm− 1) in IR spectra, an increase in DNA in both Raman (1083 cm− 1) and IR spectra (1080 cm− 1). Regarding ciprofloxacin, an increase in nucleic acids (775, 1478, 1578 cm− 1) in Raman spectra and in protein bands (1230, 1520, 1630 cm− 1), in DNA (1080 cm− 1) in IR spectra were detected. Clear discrimination of antibiotic-treated samples compared to the control was recorded, showing that Raman and IR spectroscopies, coupled to principal component analysis for data, could be used to detect molecular modifications in bacteria exposed to different classes of antibiotics. These findings contribute to the understanding of the mechanisms of action of antibiotics in bacteria.
Mots-clés Agrovoc : antibiotique, Escherichia coli, céfotaxime, spectroscopie, adn, méthode statistique, Résistance aux antibiotiques
Classification Agris : U30 - Méthodes de recherche
L73 - Maladies des animaux
000 - Autres thèmes
Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes
Auteurs et affiliations
- Nguyen Ngoc Thanh Xuan, Nong Lam University (VNM)
- Sarter Samira, CIRAD-PERSYST-UMR ISEM (FRA)
- Nguyen N. Hai, Nong Lam University (VNM)
- Daniel Philippe, IMMM (FRA)
Source : Cirad-Agritrop (https://agritrop.cirad.fr/584260/)
[ Page générée et mise en cache le 2024-04-20 ]