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Assessment of gene flow between Gossypium hirsutum and Gossypium herbaceum: Evidence of unreduced gametes in the diploid progenitor

Montes Emilie, Coriton Olivier, Eber F., Huteau Virginie, Lacape Jean-Marc, Reinhardt C., Marais Diana, Hofs Jean-Luc, Chèvre Anne-Marie, Pannetier Catherine. 2017. Assessment of gene flow between Gossypium hirsutum and Gossypium herbaceum: Evidence of unreduced gametes in the diploid progenitor. G3 - Genes Genomes Genetics, 7 (7) : pp. 2185-2193.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q2, Sujet : GENETICS & HEREDITY

Abstract : In the framework of a gene flow assessment, we investigated the natural hybridization rate between Gossypium hirsutum (AADD genome) and G. herbaceum (AA genome). The latter species, a diploid progenitor of G. hirsutum, is spontaneously present in South Africa. Reciprocal crosses were performed without emasculation between G. herbaceum and G. hirsutum. Neither examination of the morphological characteristics nor flow cytometry analysis of the 335 plants resulting from the G. hirsutum x G. herbaceum cross showed any hybrid features. Of the 148 plants produced from the G. herbaceum x G. hirsutum cross, three showed a hybrid phenotype, and their hybrid status was confirmed by SSR markers. Analysis of DNA content by flow cytometry and morphological traits clearly showed that two of these plants were triploid (AAD). The third plant had a flow cytometry DNA content slightly higher than G. hirsutum. In addition, its morphological characteristics (plant architecture, presence and size of petal spots, leaf shape) led us to conclude that this plant was AAAD thus resulting from fertilization with an unreduced AA gamete of the female G. herbaceum parent. Fluorescent In Situ Hybridization (FISH) and meiotic behavior confirmed this hypothesis. To the best of our knowledge, this is the first description of such gametes in G. herbaceum, and it opens new avenues in breeding programs. Furthermore, this plant material could provide a useful tool for studying the expression of genes duplicated in the A and D cotton genome. (Résumé d'auteur)

Mots-clés Agrovoc : Gossypium hirsutum, Gossypium herbaceum, Hybridation artificielle, Flux de gènes, Reproduction sexuée, Gamète, Polyploïdie, ADN

Mots-clés géographiques Agrovoc : Afrique du Sud, Inde

Mots-clés libres : Flux de gènes, Cotonnier, Gossypium herbaceum

Classification Agris : F30 - Plant genetics and breeding
F63 - Plant physiology - Reproduction

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Montes Emilie, INRA (FRA)
  • Coriton Olivier, INRA (FRA)
  • Eber F., INRA (FRA)
  • Huteau Virginie, INRA (FRA)
  • Lacape Jean-Marc, CIRAD-BIOS-UMR AGAP (FRA)
  • Reinhardt C., University of Pretoria (ZAF)
  • Marais Diana, University of Pretoria (ZAF)
  • Hofs Jean-Luc, CIRAD-PERSYST-UPR AIDA (FRA)
  • Chèvre Anne-Marie, INRA (FRA)
  • Pannetier Catherine

Source : Cirad-Agritrop (https://agritrop.cirad.fr/584593/)

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