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Rubber particle proteins HbREF and HbSRPP interact differently with native lipids extracted from RRIM600 H. brasiliensis latex

Wadeesirisak Kanthida, Castano Sabine, Berthelot Karine, Vaysse Laurent, Peruch Frédéric, Bonfils Frédéric, Liengprayoon Siriluck, Bottier Céline, Lecomte Sophie. 2017. Rubber particle proteins HbREF and HbSRPP interact differently with native lipids extracted from RRIM600 H. brasiliensis latex. . Edinburgh : s.n., 1 poster European Biophysical Societies' Association (EBSA) Congress. 11, Edinburgh, Royaume-Uni, 16 July 2017/20 July 2017.

Poster
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Poster_Prog_Final.pdf

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[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
2017_07_Poster_EBSA_Wadeesirisak.pdf

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Abstract : Thailand is the 1st world producer of natural rubber (NR) which is produced from the latex exuding from Hevea brasiliensis tree in the form of rubber particles suspended in serum. Latex tapped from Hevea brasiliensis (rubber tree) contains rubber particles dispersed in C-serum. Two major proteins are abundant at the surface of Hevea rubber particles namely, the HbREF and the HbSRPP, which are mainly located at the surface of large rubber particles and small rubber particles, respectively. Lipids extracted from rubber particles include phospholipids (PL), glycolipids (GL) and neutral lipids (NL) [1]. We used Brewster angle microscopy and PM-IRRAS to investigate the interactions between recombinant HbREF and HbSRPP with native lipids extracted and purified from Hevea RRIM600 latex [2] collected in the Chanthaburi province, Thailand. Native lipids from Hevea latex (clone RRIM600) were extracted and separated into three independent pure classes of lipids, including phospholipids, glycolipids and neutral lipids. Neutral lipids are the major lipids found in the extractable lipids from latex (45% w/w), followed by PL (32% w/w) and GL (23% w/w). [3] Phospholipids are mainly zwitterionic with high content of PC and PE. Monolayers of native phospholipids, glycolipids and neutral lipids were successfully formed at the air-water interface. (Résumé d'auteur)

Classification Agris : F60 - Plant physiology and biochemistry
Q60 - Processing of non-food or non-feed agricultural products

Auteurs et affiliations

  • Wadeesirisak Kanthida, CIRAD-PERSYST-UMR IATE (FRA)
  • Castano Sabine, CNRS (FRA)
  • Berthelot Karine, CNRS (FRA)
  • Vaysse Laurent, CIRAD-PERSYST-UMR IATE (THA)
  • Peruch Frédéric, CNRS (FRA)
  • Bonfils Frédéric, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0001-7016-4770
  • Liengprayoon Siriluck, Kasetsart University (THA)
  • Bottier Céline, CIRAD-PERSYST-UMR IATE (THA) ORCID: 0000-0002-5318-4275
  • Lecomte Sophie, CNRS (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/585167/)

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