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Comparison of the efficiency of different newcastle disease virus reverse genetics systems

Liu Haijin, Servan de Almeida Renata, Gil Patricia, Albina Emmanuel. 2017. Comparison of the efficiency of different newcastle disease virus reverse genetics systems. Journal of Virological Methods, 249 : pp. 111-116.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q3, Sujet : BIOCHEMICAL RESEARCH METHODS / Quartile : Q4, Sujet : VIROLOGY

Abstract : Rescue of negative-sense single-stranded RNA viruses ((−)ssRNA virus), generally requires the handling of a large number of plasmids to provide the virus genome and essential components for gene expression and genome replication. This constraint probably renders reverse genetics of (−)ssRNA virus more complex and less efficient. Some authors have shown that the fewer the plasmids, the more efficient reverse genetics is for segmented RNA virus. However, it is not clear if the same applies for (−)ssRNA, such as Newcastle disease virus (NDV). To address this issue, six variants of NDV reverse genetic systems were established by cloning combinations of NP, P and L genes, mini-genome or full-genome in 4, 3, 2 and 1 plasmid. In terms of mini-genome and full-genome rescue, we showed that only the 2-plasmid system, assembling three support plasmids together, was able to improve the rescue efficiency over that of the conventional 4-plasmid system. These results may help establish and/or improve reverse genetics for other mononegaviruses. (Résumé d'auteur)

Mots-clés libres : Newcastle, Reverse genetics

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Liu Haijin, CIRAD-BIOS-UMR ASTRE (FRA)
  • Servan de Almeida Renata, CIRAD-BIOS-UMR ASTRE (FRA)
  • Gil Patricia, CIRAD-BIOS-UMR ASTRE (FRA)
  • Albina Emmanuel, CIRAD-BIOS-UMR ASTRE (GLP)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/585512/)

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