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New multilocus variable-number tandem-repeat analysis (MLVA) scheme for fine-scale monitoring and microevolution-related study of Ralstonia pseudosolanacearum phylotype I populations

Guinard Jérémy, Latreille Anne, Guérin Fabien, Poussier Stéphane, Wicker Emmanuel. 2017. New multilocus variable-number tandem-repeat analysis (MLVA) scheme for fine-scale monitoring and microevolution-related study of Ralstonia pseudosolanacearum phylotype I populations. Applied and Environmental Microbiology, 83 (5):e03095, 16 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : MICROBIOLOGY

Abstract : Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is considered one of the most harmful plant diseases in the world. Special attention should be paid to R. pseudosolanacearum phylotype I due to its large host range, its worldwide distribution, and its high evolutionary potential. So far, the molecular epidemiology and population genetics of this bacterium are poorly understood. Until now, the genetic structure of the RSSC has been analyzed on the worldwide and regional scales. Emerging questions regarding evolutionary forces in RSSC adaptation to hosts now require genetic markers that are able to monitor RSSC field populations. In this study, we aimed to evaluate the multilocus variable-number tandem-repeat analysis (MLVA) approach for its ability to discriminate genetically close phylotype I strains and for population genetics studies. We developed a new MLVA scheme (MLVA-7) allowing us to genotype 580 R. pseudosolanacearum phylotype I strains extracted from susceptible and resistant hosts and from different habitats (stem, soil, and rhizosphere). Based on specificity, polymorphism, and the amplification success rate, we selected seven fast-evolving variable-number tandem-repeat (VNTR) markers. The newly developed MLVA-7 scheme showed higher discriminatory power than the previously published MLVA-13 scheme when applied to collections sampled from the same location on different dates and to collections from different locations on very small scales. Our study provides a valuable tool for fine-scale monitoring and microevolution-related study of R. pseudosolanacearum phylotype I populations. (Résumé d'auteur)

Mots-clés Agrovoc : Ralstonia solanacearum, Ralstonia, Technique analytique, Génétique des populations, Phylogénie, Polymorphisme génétique, Marqueur génétique, Identification, Surveillance épidémiologique

Mots-clés géographiques Agrovoc : Martinique, Réunion, Cameroun, Côte d'Ivoire, Guyane française, Afrique du Sud, Taïwan

Mots-clés complémentaires : Ralstonia pseudosolanacearum

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Agence(s) de financement européenne(s) : European Regional Development Fund

Auteurs et affiliations

  • Guinard Jérémy, CIRAD-BIOS-UMR PVBMT (REU)
  • Latreille Anne
  • Guérin Fabien, Université de la Réunion (REU)
  • Poussier Stéphane, Université de la Réunion (REU)
  • Wicker Emmanuel, CIRAD-BIOS-UMR IPME (FRA) ORCID: 0000-0003-0927-7404

Source : Cirad-Agritrop (https://agritrop.cirad.fr/585758/)

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