Root cone angle is enlarged in docs1 LRR- RLK mutants in rice

Bettembourg Mathilde, Dal-Soglio M., Bureau Charlotte, Vernet Aurore, Dardou Audrey, Portefaix Murielle, Bes Martine, Meynard Donaldo, Mieulet Delphine, Cayrol Bastien, Périn Christophe, Courtois Brigitte, Ma J.F., Dievart Anne. 2017. Root cone angle is enlarged in docs1 LRR- RLK mutants in rice. Rice, 10:50, 8 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
Published version - Anglais
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Root cone angle is enlarged in docs1 LRR-RLK mutants in rice Bettembourg_et_al-2017-Rice.pdf

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Quartile : Q1, Sujet : AGRONOMY

Abstract : Background: The DEFECTIVE IN OUTER CELL LAYER SPECIFICATION 1 (DOCS1) gene belongs to the Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) subfamily. It has been discovered few years ago in Oryza sativa (rice) in a screen to isolate mutants with defects in sensitivity to aluminum. The c68 (docs1–1) mutant possessed a nonsense mutation in the C-terminal part of the DOCS1 kinase domain. Findings: We have generated a new loss-of-function mutation in the DOCS1 gene (docs1–2) using the CRISPR-Cas9 technology. This new loss-of-function mutant and docs1–1 present similar phenotypes suggesting the original docs1–1 was a null allele. Besides the aluminum sensitivity phenotype, both docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers. Moreover, our new results suggest that DOCS1 could also play a role in root cap development. We hypothesized these docs1 root phenotypes may affect gravity responses. As expected, in seedlings, the early gravitropic response was delayed. Furthermore, at adult stage, the root gravitropic set angle of docs1 mutants was also affected since docs1 mutant plants displayed larger root cone angles. Conclusions: All these observations add new insights into the DOCS1 gene function in gravitropic responses at several stages of plant development. (Résumé d'auteur)

Mots-clés Agrovoc : Oryza sativa, Système racinaire, Mutant, Gravité, Tropisme, Aluminium, Gène, Expression des gènes, Génie génétique, Stade de développement végétal, Anatomie végétale, Morphologie végétale, Racine

Mots-clés libres : Rice, Root cap, Angle, Gravitropism, Docs1, Lrr, Rlk, GSA, RCA, RGA

Classification Agris : F30 - Plant genetics and breeding
F62 - Plant physiology - Growth and development
F50 - Plant structure

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Bettembourg Mathilde, CIRAD-BIOS-UMR AGAP (FRA)
  • Dal-Soglio M., Université de Montpellier (FRA)
  • Bureau Charlotte, CIRAD-BIOS-UMR AGAP (FRA)
  • Vernet Aurore, CIRAD-BIOS-UMR AGAP (FRA)
  • Dardou Audrey, CIRAD-BIOS-UMR AGAP (FRA)
  • Portefaix Murielle, INRA (FRA)
  • Bes Martine, CIRAD-BIOS-UMR AGAP (FRA)
  • Meynard Donaldo, CIRAD-BIOS-UMR AGAP (FRA)
  • Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
  • Cayrol Bastien, Université de Montpellier (FRA)
  • Périn Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-2469-310X
  • Courtois Brigitte, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-2118-7102
  • Ma J.F., Okayama University (JPN)
  • Dievart Anne, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-9460-4638 - auteur correspondant

Source : Cirad-Agritrop (

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