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Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system

Nieves-Cordones Manuel, Mohamed Sonia, Tanoi Keitaro, Kobayashi Natsuko I., Takagi Keiko, Vernet Aurore, Guiderdoni Emmanuel, Périn Christophe, Sentenac Hervé, Very Anne-Aliénor. 2017. Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system. Plant Journal, 92 (1) : pp. 43-56.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Outlier, Sujet : PLANT SCIENCES

Abstract : The occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm), cesium (Cs+) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs+ from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs+ content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side-effects. Conversely, the development of biotechnological approaches aimed at reducing Cs+ accumulation in rice remain challenging. Here, we show that inactivation of the Cs+-permeable K+ transporter OsHAK1 with the CRISPR-Cas system dramatically reduced Cs+ uptake by rice plants. Cs+ uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs+ uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs+ and K+; and (ii) a high capacity to transport Cs+ from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with 137Cs+, plants lacking OsHAK1 function displayed strikingly reduced levels of 137Cs+ in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents. (Résumé d'auteur)

Mots-clés Agrovoc : Riz, Oryza sativa, Contamination radioactive, Césium, Absorption, Sol pollué, Physiologie végétale

Mots-clés géographiques Agrovoc : Japon

Classification Agris : T01 - Pollution
Q03 - Food contamination and toxicology
F60 - Plant physiology and biochemistry
F01 - Crop husbandry

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Nieves-Cordones Manuel, INRA (FRA)
  • Mohamed Sonia, CIRAD-BIOS-UMR AGAP (FRA)
  • Tanoi Keitaro, University of Tokyo (JPN)
  • Kobayashi Natsuko I., University of Tokyo (JPN)
  • Takagi Keiko, University of Tokyo (JPN)
  • Vernet Aurore, CIRAD-BIOS-UMR AGAP (FRA)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Périn Christophe, CIRAD-BIOS-UMR AGAP (FRA)
  • Sentenac Hervé, INRA (FRA)
  • Very Anne-Aliénor, INRA (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/586754/)

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