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Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds

Dussert Stéphane, Serret Julien, Bastos-Siqueira Aldecinei, Morcillo Fabienne, Dechamp Eveline, Rofidal Valérie, Lashermes Philippe, Etienne Hervé, Joët Thierry. 2018. Integrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds. Journal of Experimental Botany, 69 (7) : pp. 1583-1597.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : The intermediate seed category was defined in the early 1990s using coffee (Coffea arabica) as a model. In contrast to orthodox seeds, intermediate seeds cannot survive complete drying, which is a major constraint for seed storage, for both biodiversity conservation and agricultural purposes. However, intermediate seeds are considerably more tolerant to drying than recalcitrant seeds, which are highly sensitive to desiccation. To gain insight into the mechanisms governing such differences, changes in desiccation tolerance (DT), hormone content and the transcriptome were analysed in developing coffee seeds. Acquisition of DT coincided with a dramatic transcriptional switch characterised by the repression of primary metabolism, photosynthesis and respiration, and the upregulation of genes coding for late embryogenesis abundant (LEA) proteins, heat shock proteins (HSP) and antioxidant enzymes. Analysis of heat-stable proteome in the mature coffee seed confirmed the accumulation of LEA proteins identified at the transcript level. Transcriptome analysis also suggests a major role for ABA and for the transcription factors CaHSFA9, CaDREB2G, CaANAC029, CaPLATZ and CaDOG-like in DT acquisition. The ability of CaHSFA9 and CaDREB2G to trigger HSP gene transcription was validated by Agrobacterium-mediated transformation of coffee somatic embryos. (Résumé d'auteur)

Mots-clés Agrovoc : Coffea arabica, Fève de café, Maturation, Transformation génétique, Agrobacterium, Tolérance à la sécheresse, Développement embryonnaire, Transcription génique, Séchage, Expression des gènes, Aptitude à la conservation, Température, Organisme génétiquement modifié, Physiologie végétale, Développement biologique

Mots-clés géographiques Agrovoc : Réunion

Mots-clés libres : Agrobacterium transformation, Coffea arabica, Desiccation tolerance, Heat-stable proteome, Intermediate seed, Late embryogenesis abundant proteins, Late maturation, Heat shock factor, Heat shock protein, Transcriptome

Classification Agris : Q60 - Processing of non-food or non-feed agricultural products
F60 - Plant physiology and biochemistry
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Dussert Stéphane, IRD (FRA)
  • Serret Julien, IRD (FRA)
  • Bastos-Siqueira Aldecinei, IRD (FRA)
  • Morcillo Fabienne, CIRAD-BIOS-UMR DIAPC (FRA)
  • Dechamp Eveline, CIRAD-BIOS-UMR RPB (FRA)
  • Rofidal Valérie, INRA (FRA)
  • Lashermes Philippe, IRD (FRA)
  • Etienne Hervé, CIRAD-BIOS-UMR IPME (FRA)
  • Joët Thierry, IRD (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/586804/)

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