Agritrop
Home

Ex vitro rooting using a mini growth chamber increases root induction and accelerates acclimatization of Kopyor coconut (Cocos nucifera L.) embryo culture-derived seedlings

Sisunandar, Alkhikmah, Husin Arief, Julianto Teguh, Yuniaty Alice, Rival Alain, Adkins Stephen. 2018. Ex vitro rooting using a mini growth chamber increases root induction and accelerates acclimatization of Kopyor coconut (Cocos nucifera L.) embryo culture-derived seedlings. In Vitro Cellular and Developmental Biology. Plant, 54 (5) : pp. 508-517.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
[img] Version Online first - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
135180

Télécharger (1MB) | Request a copy
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
a-rival-2018.pdf

Télécharger (5MB) | Request a copy

Quartile : Q3, Sujet : PLANT SCIENCES / Quartile : Q4, Sujet : DEVELOPMENTAL BIOLOGY / Quartile : Q4, Sujet : CELL BIOLOGY

Abstract : Ex vitro rooting using a mini growth chamber to maintain relative humidity has been used to mass produce true-to-type Kopyor coconut (Cocos nucifera L.) seedlings through embryo culture. This new process was found to (1) improve the proportion of seedlings successfully transferred to soil (from 40 to 90% for seedlings with roots); (2) achieve this step in the shortest time possible (a reduction from 10 to 4 mo in in vitro culture); (3) improve root formation using indolebutyric acid (IBA; an improvement in the number of primary roots from 2 to 5); and (4) improve the vigor of seedlings ex vitro (improvements of fresh weight, shoot length, number of opened leaves, leaf thickness, amount of epicuticular wax, and stomatal density). The best ex vitro rooting and rapid acclimatization protocol was obtained when 4-mo-old seedlings with two opened leaves were kept in the mini growth chamber for 3 mo before being transferred into soil, and when the mini growth chamber was flooded with a quarter strength hybrid embryo culture (HEC) medium with 1 μM IBA but depleted of vitamins and sugar. This protocol was efficient in delivering high-value Kopyor seedlings to the field (90% success rate), with a decreased risk of contamination and lower labor cost. The improved process was found applicable to both tall and dwarf Kopyor and other coconut types.

Mots-clés Agrovoc : Cocos nucifera, Culture d'embryon, Expérimentation en laboratoire, Chambre de croissance, Morphologie végétale, AIB, Enracinement, Adaptation, Physiologie végétale

Mots-clés géographiques Agrovoc : Java, Madura, Sumatra, Indonésie

Mots-clés libres : Acclimatization, Coconut, Embryoculture, Kopyor

Classification Agris : F62 - Plant physiology - Growth and development

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Sisunandar, University of Purwokerto (IDN) - auteur correspondant
  • Alkhikmah, University of Purwokerto (IDN)
  • Husin Arief, University of Purwokerto (IDN)
  • Julianto Teguh, University of Purwokerto (IDN)
  • Yuniaty Alice, Jenderal Soedirman University (IDN)
  • Rival Alain, CIRAD-DRS (IDN) ORCID: 0000-0001-6304-3268
  • Adkins Stephen, University of Queensland (AUS)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587389/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2020-10-14 ]